What could be the biotin: avidin ratio taken for their interaction study using an electrochemical system?

Jinsa Paul @Jinsa-Paul

19 June 2023 1 3K Report

I have 1 mg of avidin and 100 mg of biotin. How do I prepare the stock solution? I want to study the interaction of these two using electrochemical workstation? Is 1:1 ratio followed?

Thaís Pedrete

You can have up to 4 biotin molecules bound to avidin.

So, depending how many molecules you want to bind, you use the molar ratio accordingly.

The concentration you prepare for biotin and avidin will depend on this molar ratio.

For example: 1:4 (avidin:biotin)...the stock solution of biotin should be 4x higher concentrated than avidin, and then you just add equal proportion of volume from each stock to the final sample.

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