Are there Religious Cognitive- Emotional Therapy assessment tools available to assess or screen patients suffering from depressive disorders?

My Research proposal topic is The efficacy of Religious Cognitive- Emotional Therapy (RCET) in Treating Depression in the district Wanica - Suriname. My Research question: "Is Religious...

23 December 2020 6,955 4 View

Do those who are uninsured in the USA receive Covid-19 inpatient treatment?

I am drafting a paper on the number of hospital beds in the different States of the US and find that half of US States have bed provision equivalent to the less developed countries. This leads to...

16 April 2020 2,775 10 View

In within-subjects regression, interaction is non-significant but one of the parameter estimates is. Unsure how to interpret?

Hello, I've been having issues interpreting the results from a set of data I have. Basically, it's a within-subjects design with two experimental conditions (Condition 1 and 2), and one continuous...

11 April 2019 2,449 2 View

Does chelex extraction prevent ethidium bromide staining of genomic DNA?

We are doing chelex extraction of human DNA from buccal cells. Before proceeding to PCR, I decided it would be a good idea to confirm extraction by running the genomic DNA through gel...

29 January 2019 8,410 2 View

In your own opinion, what are the effects of integrating your local industry to your basic education?

One of our local industry is citrus fruits production. In our university, we have food and natural products technology centers that process these citrus fruits into wines, jam, and soap among...

13 November 2018 1,175 3 View

We have a free webinar coming up on March 28 that might be of interest to some of you. Identification and Characterization of Neural Stem Cells: Why i...

We have a free webinar coming up on March 28 that might be of interest to some of you. Identification and Characterization of Neural Stem Cells: Why is This so Darned Difficult? Presented by...

02 March 2012 4,109 6 View

Science experiment from June 2019 necessitates re-assessment of quantum mechanics - it's not probabilistic but deterministic

Science moves very rapidly sometimes. Current astrophysics courses are built on the nearly-century-old tradition of interpreting quantum mechanics in terms of probability. However, in 2019 it was...

01 January 1970 6,261 3 View

Using the cosmological constant, more maths and Special Relativity in relation to the Robertson-Walker metric and the Big Bang's expanding universe

01 January 1970 675 1 View

Intergalactic and time travel via division by zero

01 January 1970 4,826 9 View

Could division by zero tell us where the universe came from?

01 January 1970 1,986 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View