Primer

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Questions related to Primer

How can I determine the strain of an albino mouse that I find randomly?

Are there any molecular techniques, e.g., sequencing, fragment length analysis, or something similar, that can be used to determine the strain of this mouse? If so, could you tell me the gene...

16 December 2024 6,366 3 View

My DNA bands have donut shape on Agarose gel electrophoresis?

Hi, I move to a new lab. And I found that my DNA bands show donut shape on Agarose gel electrophoresis. I can't slove this problem. I use 1X TAE buffer. Run 100 V for 30 min. Post-staining gel...

16 December 2024 1,180 0 View

Please identify this parasite that was found inside a carp?

Parasite found inside carp

15 December 2024 3,511 1 View

Do you know all of conditions for formation of bentonites?

I want to know more about bentonites.

14 December 2024 4,815 2 View

How can I apply different seeds on different edges of a partitioned cube?

I have a cube that I want to mesh in a certain way. The first picture is the reference mesh that I want to replicate. I have partitioned my model according to the reference model. But when I try...

13 December 2024 3,907 0 View

How can managers leverage AI and big data to improve decision-making and organizational efficiency?

This question explores how managers can utilize modern technologies, such as artificial intelligence and big data, as strategic tools to support decision-making processes within organizations. It...

12 December 2024 3,418 4 View

What should be done to reduce the absorption of methylene blue dye by vanadia instead of degradation it?

What should be done to reduce the absorption of methylene blue dye by vanadia instead of degradation it? During the destruction of methylene blue, after one hour of darkness, the color of...

12 December 2024 859 0 View

Is there any way of inhibiting Parvalbumin interneuron activity?

We are looking to selectively inhibit the activity of specific interneuron subtypes in the DG. The obvious method would be Targeting DREADDS through cre lox recombination, but the caveat is that...

07 December 2024 8,705 0 View

I use AQ97 High Fidelity DNA Polymerase enzyme and rice DNA for PCR but the gel bands are fade, how can i improve the yield?

30μl reaction components include, DNA 2μl, Master 15μl, Primer 0.7+0.7μl, water 11.6μl PCR program 98°C 2min 1 98°C 15S 35 56-61°C 20S 35 72°C 30S 35 72°C...

04 December 2024 6,826 11 View

How could positive real-time PCR results be negative after repeating the run? It's an error or possible?

I repeated the amplification of the same extracted DNA and got different results! PS: I used the same probe, primers, and master mix, and all the conditions were the same for both runs.

03 December 2024 8,092 3 View

Please help me publish my article in the journal ?

We are a Chinese education company dedicated to helping our clients publish SCI, SCIE, SSCI WoS indexes quickly. We have a wide range of paper orders in various fields and we are actively looking...

02 December 2024 2,541 1 View

To find articles from Paleontographica B?

Dear colleagues, Could anybody by chance provide me with pdfs of these papers? Regards, Natalia Zavialova Antonescu, E., & Lantz, J. T. (1973). Considérations sur des Mégaspores et...

20 November 2024 6,973 1 View

Does non-random recombination with respect to allelic identity exist?

As part of an upcoming book on adaptive evolution, I place a lot of emphasis on whether an evolutionary process acts random or non-random. This makes a lot of different, think about the...

16 November 2024 3,083 2 View

How the FRET energy by the acceptor molecule is utilized? Is there any quantitative relationship to find out this?

In case of QD film, FRET phenomenon occur , Donor QD transfer energy to acceptor QD molecule. How that transferred energy is utilized by the acceptor molecule? is there any quantitative relation...

14 November 2024 7,409 4 View

Do you know highest grade Fe mine in world?

I want to know more about Fe ore deposits in world.

14 November 2024 7,067 1 View

Neutralization antibody staining with virus?

I'm currently setting up an focus reducation neutralization test(FRNT) experiment for RSV. The focus is coming out well, but there's one issue: in the group with serum containing the neutralizing...

13 November 2024 2,016 0 View

How long can I store methanolic extracts of the pine needles of Pinus kesiya?

We will be conducting an experiment on the use of Pinus kesiya as an alternative pesticide for Brassica rapa. We will extract the pine needles using methanol, however we do not know how long can...

13 November 2024 5,282 3 View

Why does my BMDM become CD11c+ without stimulation?

Hi, I have been trying to establish primary BMDM from C57 mice. We have consistently found our macrophages are CD11b+ F480+ CD11c+. In our protocol, we seed cells at 0.5mil cells/cm2 (4.75 mil...

13 November 2024 3,101 0 View

In vitro transcription query?

why am I getting to see 2 RNA bands (one expected and one extraneous) after performing In vitro transcription reaction with T7 RNA polymerase using HindIII linearised DNA as a template. Even after...

11 November 2024 8,089 2 View

Chemistry of two misleading vegetables and an apology?

Can I first apologise for being unable to send the authors a thank-you message after receiving some wonderful papers. Apparently I can't thank an author unless it was the same one I approached...

10 November 2024 1,420 2 View

MHV Plaque Assay- Plaque formation appears odd?

Hello, I recently did a plaque assay using MHV A59. L929 cells were exposed to the virus for 1 hour, with slight plate tilting every 15 minutes. I added 1% agarose directly to the diluted virus...

05 November 2024 5,162 1 View

Treatment of diabetes and unani medicine?

which drugs are available in unani medicine , what is rational

05 November 2024 5,455 2 View

I have prepared 1mg/ml solution of a nanomaterial, how could I load 500microlitre of solution on filter paper disc to study antimicrobial activity?

To study anti microbial activity of nanomaterials.

04 November 2024 5,364 4 View

Is a specialized electrophoresis tank essential for Comet Assay, or can standard tanks suffice?

I’m planning to run the Comet Assay to assess DNA damage, but I’m debating whether it’s worth investing in a specialized electrophoresis tank or if a standard tank can handle it well enough. Has...

31 October 2024 5,337 4 View

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