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Questions related to Primer
Is the V4 region of the 16S rRNA gene better sequenced at 1x 300bp or 2x 150bp.
21 June 2023 4,228 3 View
Which abiotic factors are necessary for plants to survive in an aquatic ecosystem and abiotic factors in an environment the biotic factors?
21 June 2023 5,012 1 View
RNA from soil will be isolated with Qiagen kit, sequencing will be fine with Illumina 2x150nt.
20 June 2023 3,763 1 View
I've done a 50 ns protein-ligand MD simulation. Getting stable RMSD for the first 20 ns, but it increases afterwards, and at some point frame, it's getting very high. My question is, is my md...
20 June 2023 1,142 3 View
Based on the Rt-PCR analysis results of the oligos I have designed, it appears that there is a consistent signal from the NTC and negative patient samples with Ct values ranging from 30 to 32....
20 June 2023 2,995 6 View
Dear all, I am working on identification of exosome related protein markers through western blot antibody probing. In my samples, there is an impurity seen at 66KDa which is obtained during the...
20 June 2023 9,322 2 View
Total genomic DNA of an organism is digested using restriction endonuclease and the fragments are inserted into a suitable phage.
20 June 2023 2,074 2 View
We plan to sequence several samples isolated from rat brain hippocampus. My previous colleagues used NextFlex version 3, which was working fine, and now we are continuing with NextFlex v4. The...
20 June 2023 3,878 0 View
I have problem about MTT assay in U87 cell line. After add MTT solution in U87 cell line, the cell were floated. Who used to find the same problem. Why is cause of this action? Please answer and...
20 June 2023 147 2 View
Dear ResearchGate Community, I am currently engaged in single-cell analysis for my research project and would greatly appreciate your insights and experiences regarding the use of Seurat and...
20 June 2023 9,043 5 View
Education sector
19 June 2023 3,153 4 View
Next-Generation Sequencing (NGS) has become an essential tool in modern life-science research, generating vast amounts of data that provide insights into genetic variation, gene expression,...
19 June 2023 1,031 0 View
Hi, I isolated genomes from different fungi. One of them is Boletus edulis (according ITS and 28srRNA primers ~99%). But sequencing of peroxidase gene, which was amplified by specific primers for...
19 June 2023 8,804 0 View
We performed restriction digestion and to analyze the restriction pattern by and received a bad result. What may be the reason behind it
19 June 2023 3,304 5 View
hi Volatile oils in flowers and butterflies?
18 June 2023 580 0 View
AS the title. The fecal samples were stored in the -80 ℃ refrigerator for several years. Want to know if it is suitable for 16sRNA or metagenomic sequencing to analyze the gut microbiota.
17 June 2023 5,074 1 View
Hello, I have a small white scientific research. How do you extract nematode DNA from soil? The literature I have seen says that there are two methods, one is to extract total soil DNA using kits...
17 June 2023 5,114 1 View
I’m purifying a recombinant protein with a Hi tag via nickel affinity chromatography. If my sample has MgSO4 in it as part of the purification process, will that interfere with nickel affinity...
16 June 2023 9,130 2 View
External validity is the extent to which we can generalize the findings of a study to other situations, people, settings, and measures.External validity can be increased by using broad inclusion...
16 June 2023 8,912 2 View
Fish farming of red tilapia ?
16 June 2023 453 2 View
Hi everyone, I´ve been struggling with low DNA yields and weird ratio readings extracted from stool samples collected from fish. I am currently using the PowerFecal Pro DNA extraction kit from...
15 June 2023 4,961 4 View
I am interested in predicting the protein structure of my protein of interest. Using NCBI BLAST, I found an experimental structure that corresponds to a domain of my protein, showing 24% query...
14 June 2023 8,779 3 View
When I load a protein sample, the sample drifts up the sides of the sample well.My Running Buffer is Tris-Tricine-Hepes system.And the sample strip will become U-shaped after starting to...
14 June 2023 3,748 5 View
We are trying to clone the 60bp shRNA related to a gene in the PCDH vector, but unfortunately we do not see the desired band in PCR. The annealing step was performed with the usual protocols....
14 June 2023 5,898 1 View