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My graduate student is using Ethovision XT v17 to measure fish movement in response to conspecific alarm cues. Fish are placed in a 37L aquarium for these trials. We also would like to measure...
10 February 2025 1,744 0 View
Are opaque walled plates used directly for seeding, or is the end product aliquoted into the opaque walled plates to record luminescence? Is there a particular wavelength or setting for measuring...
09 February 2025 1,369 1 View
I transfected SARS-CoV-2 Spike (https://www.addgene.org/141382/) in HEk293T cells and 48 h later collected cell lysate in Pierce lysis buffer containing protease inhibitor cocktail. However, upon...
08 February 2025 1,265 2 View
I ran MD of a Prot-Lig complex for 200ns. Then proceeded to GBSA calculation using gmx_MMPBSA. Start frame = 0 and Endframe = 20000; interval = 50 (in mmpba.in) The entropy curve for the full...
06 February 2025 5,922 0 View
Dear Research Community, I am modelling the stress-strain behaviour of FRP-confined concrete in ABAQUS. I have written a USDFLD sub-routine. My ABAQUS stress-strain curve follows the expected...
05 February 2025 2,282 2 View
I’ve been trying to calculate relative telomere length using Cawthon’s monochrome multiplex PCR method on a Bio-Rad CFX96™ Real-Time PCR Detection System. However, when I attempt to run the PCR,...
04 February 2025 648 1 View
I'm going to express recombinant spidroin from a pET-22b(+) plasmid, the protein MW is around 51 kDa and I want to study where the protein is expressed (extracellular, intracellular...
02 February 2025 5,640 3 View
I am currently seeking an immortalized bovine intestinal cell line for research purposes. If anyone has such a cell line available or knows someone who may be willing to share it, I would greatly...
31 January 2025 2,618 0 View
Hi, I am investigating an effect of a protein on mTORC1 signaling via western blot and probing for PS6K and P4EBP1, the best known mTORC1 substrates, as well as totals S6K-4EBP1, over expressed...
30 January 2025 7,235 3 View
By knowing the root secretions, it is possible to identify the microbial species.
26 January 2025 8,358 4 View
explain your idea
24 January 2025 8,359 2 View
What are the different measures to identify the grass?
18 January 2025 8,478 5 View
Can we validate the models used via the Lagrange interpolating polynomial? and how proud the novel values of target
17 January 2025 1,790 2 View
I am looking for any studies that provide information on the potential (or lack thereof) of using the ice slurry method for anaesthesia in small fish.
16 January 2025 5,607 0 View
Literature said it would be either 1:1 or 5:1 or some other ratios are also mentioned. what are the reason behind this ratio?
12 January 2025 300 1 View
Hi, I am doing cell viability test with U2OS cells, and I want to evaluate co-incubation with experimental compounds (autophagy inhibitors) and doxorrubicin, so Im not able to use priopidium...
09 January 2025 9,397 0 View
I am working with phototrophic biofilms and would like to cryopreserve them for future experiments. My primary goal is to maintain the viability and structural integrity of the biofilm, as well as...
08 January 2025 9,979 0 View
In case of diabetes, there were several evidence of prolonged inflammation due to interruption of m1 to m2 macrophage polarization. So if anyone want to promote wound healing in case of diabetic...
06 January 2025 3,654 1 View
Creutzfeldt–Jakob disease and other prion diseases
06 January 2025 3,405 1 View
How can I determine the governing equations for these processes, and what bibliography would you recommend for identifying the most accessible simulation solutions to address how the additional...
04 January 2025 7,976 2 View
Prp treatment in endometritis
02 January 2025 1,834 2 View
I am using Qiagen Plant DNA kit for extracting Tepary bean (Phaseolus acutifolius) DNA. For 260/280, I am getting a ratio between 1.71 - 1.84 and concentration between 50 - 200ng/µL
29 December 2024 7,135 1 View
If you can, please share to this email address ([email protected]) Regards
29 December 2024 7,518 0 View
1. What would be the ideal control for these assays like DPPH and ABTS? while go through various literature, i noticed some authors use growth medium (MRS broth) as a control, others use water as...
27 December 2024 826 0 View