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Questions related to Primer
Could you please send a few articles on management with mixed-model research?
02 June 2023 2,949 3 View
I want to collect all the available NGS data of a bacterial strain collected from a certain country, how can I do that?
02 June 2023 4,359 2 View
I need to quantify intracellular calcium, but do not have fura-2am or different calcium influx assay, so methylthymol blue or O-cresolphthalein are the options at hand, then perform colorimetric...
01 June 2023 5,844 1 View
If anyone has used ABTS peroxidase in ELISA as the substrate, how long do you incubate before you stop/read the plates? I am going to develop an ELISA and plan to use ABTS peroxidase and aim to...
01 June 2023 1,407 4 View
Gnathia is a genus of marine isopod crustaceans.
01 June 2023 9,872 2 View
Also, the transference number and transport number are same or different? If they are different then how to calculate them for cations and anions?
01 June 2023 3,701 1 View
Someone claimed my article as their own on Research Gate. I reported the profile and reached out to customer support, but I see no redress.
01 June 2023 123 3 View
I need a barcod primers for bacterial identification from blood samples
31 May 2023 5,967 1 View
Which tools or methods are used to compare the relative gene expression of real-time PCR value other than the Livak method?? I have values of CQ of both gene and internal reference. I want to know...
31 May 2023 6,234 2 View
My advisor suggested that I use the recombinant protein from the elisa kit as a positive control but I can't find any reference to know how much protein to put in each well...
30 May 2023 6,148 4 View
I used KAPA hifi pcr kit to linearize the plasmid for in-fusion cloning. Following the protocol, the DNA template amount was 5 ng and I already diluted it 100x. Annealing temperature: 66 69. The...
30 May 2023 5,207 5 View
I have tried using Autodock to dock the Tio2 complex to a protein. However, a few of the web-based properties of this metal ion need to be validated. The computer algorithm identifies it as a...
30 May 2023 9,074 4 View
I have laryngeal cancer samples from which I am to extract DNA/RNA and Protein. However, the kit I have only extracts DNA and RNA (Qiagen AllPrep DNA/RNA Kit), and does not extract protein. How do...
29 May 2023 9,915 6 View
Introduction: In recent years, the veterinary industry has witnessed a surge in entrepreneurial ventures focused on transforming the way we care for our animal companions. In this blog post, we...
29 May 2023 3,113 0 View
I have worked with two primers: LCO1490/HCO2198 and dgLCO1490/dgHCO2198 for the COI gene in the same specie. But I get different information using both.
29 May 2023 6,788 2 View
I am trying to perfom a sample size calculation for neuroimaging study. Specifically, the participants, after filling questionnaire yileding a spefic score, perform a task in three different...
28 May 2023 9,982 1 View
I want to check cell cycle phase specific expression of a gene but do not want to go for Flow Cytometry. I want an alternative strategy for cell cycle phase determination. I found some drugs that...
28 May 2023 8,996 1 View
When purifying my DNA amplicons, I need to excise my DNA of interest from my gel (1.2% agarose). I've observed that the DNA is distributed top and bottom of the thickness. Out of curiosity, I've...
27 May 2023 7,159 6 View
Hi, Could somebody please explain to me why lunar "soil" simulants JSC-1 and JSC-1A contain about 3% of ferric oxide while real lunar regolith do not have any ferric iron? Thank you, Igor Brown, PhD
27 May 2023 5,458 3 View
Hello everybody, I'm a master degree student. I'm working with 16S data on some environmental samples. After all the cleaning, denoising ecc... now I have an object that stores my sequences,...
26 May 2023 6,379 2 View
Dear researchers, I have performed a cloning study for a gene 3550 bp but it didn't work. Firstly I gel-extracted the gene after digesting the vector with two restriction enzymes. I have also...
26 May 2023 9,558 5 View
I am doing HEK293 antibiotic selection after transfection, and everyday i find dead cells collected at the center of the well, although the plate is kept static in the incubator. Any plausible...
26 May 2023 4,469 4 View
For droplet-based assays, such as digital-droplet PCR or similar, it is common to image/process only a selection of the generated droplets. Conceptually, I would think that increasing the number...
26 May 2023 1,962 1 View
because the reproducibility of the molecular dynamics results quite changes because of initial velocities and other factors.
26 May 2023 8,851 1 View