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Is there any SCI journal on machine learning that charges no APC fees ?
11 July 2024 1,482 0 View
We are trying to isolate RNA from frozen samples in alginate scaffolds with Trizol. We tried to increase the pH to speed up the dissolution of alginate. At pH 9, the most RNA was isolated, but the...
11 July 2024 6,933 2 View
I need to isolate lectin from crabs, as per protocols all are using either sepharose 4B or sepharose CL 4B (adsorption chromatography). But these two types are not available with me, instead of...
09 July 2024 4,944 0 View
Hello everyone, I am try to amplify reverse transcription product (cDNA) from totalRNA. I can only obtain short sequences (as shown in gel image attached). The peak is around 400-500 bp. However,...
09 July 2024 304 5 View
I have input the pdb file combining both protein and ligand in it and I am getting this error repeatedly. Kindly help me with this.
08 July 2024 9,086 2 View
First, I performed co-transformation using an electroporator device and then picked 15 colonies and cultured them overnight in LB media containing the antibiotic that the recombinant plasmid is...
03 July 2024 10,048 1 View
KNApSAcK database list several herb ingredients as "herba". I want to ensure whether it means "whole plant" or just "aerial part" of the plant.
02 July 2024 9,493 1 View
I want to use a DAB dye to detect hydrogen peroxides in plant tissues, so I ordered the following product: https://www.sigmaaldrich.com/DE/de/product/aldrich/d12384 The problem is that the DAB...
02 July 2024 5,948 0 View
DOI: 10.13140/RG.2.2.33214.09284 15 June 2048 equals my extrapolated year date of planets nonviable conclusion. 120º Celsius However, 01/05/2047 is my extrapolated point of the beginning of PONR...
02 July 2024 2,216 0 View
I am currently studying the detection of Pb and Cd using SPGE. The following parameters have been used i.e. deposition potential of -1.4 V, deposition time of 200 s, step increment of 5 mV, pulse...
26 June 2024 8,024 0 View
Is it Indexed in SCOPUS or Web of Science? Can it be considered equivalent to SCOPUS Journal Publication?
19 June 2024 9,235 0 View
I am trying to submit a multiple alignment sequence for the rbcL gene region in Genbank. In total I have 71 sequences and 3 of these sequences have a singular internal stop codon somewhere within...
18 June 2024 7,078 1 View
The standard power used in photocatalysis is 100mW/cm2. But no one really mentions what wavelength the power meter generally set to measure the intensity. I have seen cases where they measure the...
18 June 2024 2,433 1 View
anyone please ?
11 June 2024 1,155 2 View
These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...
07 June 2024 2,604 3 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
06 June 2024 777 2 View
I am a master student and writing research paper. I have 3 yes/no questions, 2 multiple responses questions and 1 Likert scale question. I would like find out whether age has relationship to my...
06 June 2024 1,905 9 View
1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.
06 June 2024 5,225 1 View
microbial contamination of food
06 June 2024 8,029 1 View
I had a valid idea for a meta-analysis involving multiple prospective & retrospective studies with no prior meta-analyses on the same chosen sub-population. When confirming the validity of my...
05 June 2024 8,788 1 View
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
04 June 2024 3,234 7 View
Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...
04 June 2024 8,081 6 View
Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....
04 June 2024 4,281 3 View
I received it from: [email protected], with the following text. Dear Author, Welcome! Your recent publication has been provisionally selected for the "Best Researcher Award."...
03 June 2024 4,028 2 View