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Questions related to Primer
An article has been successfully peer reviewed and published online but resubmitted in a society conference proceedings for presentation. Does such practice ethical or unethical?
11 January 2024 1,932 3 View
When I overexpressed a protein in a cell line, I found decrease of phospho-PLC (phospholipase C) at the protein level through WB. But when I knocked out the protein, there was no difference of...
11 January 2024 8,222 3 View
As i culture my cells, i noticed that some of them got bigger and round.The background is clear, and the cell growth rate is fine.Can someone please tell me why the cell morphology changes?
11 January 2024 3,480 4 View
I have used the CTAB extraction method (with Lithium chloride) for RNA extraction from coconut leaves CTAB method (Mosr et al 2004, Jakola et al 2001) 1. 1 g sample + 10 mL CTAB (65°C) in 50 mL...
11 January 2024 7,208 0 View
Hi everyone, I will soon start setting up the cuprizone model of demyelination/multiple sclerosis. Cuprizone is purchased from Sigma in powder form and mixed with regular rodent chow (0.2% w/w)....
10 January 2024 4,317 0 View
Has anyone used Promega's bioluminescent MTase-Glo Methyltransferase Assay kit on 96-well plates? I am looking for a protocol that calculates the amount of individual reagents for this type of...
10 January 2024 8,605 0 View
Further research on the use of vitreous humor fluid in promoting wound healing in cattle could prove to be a valuable addition to veterinary medicine.
10 January 2024 4,257 1 View
Good afternoon all. I'm a PhD student from India currently working on scrub typhus disease investigation. I extracted DNA from blood samples using Qiagen blood extraction kit and screened by using...
10 January 2024 6,828 0 View
hoe can i rectify the title shown in google as ORTHODONTIC CAMOUFLAGE OF SKELETAL CLASS II MALOCCLUSION USING MINI-IMPLANTS- A CASE REPORT from current title as INTERNATIONAL JOURNAL OF SCIENTIFIC...
09 January 2024 3,901 2 View
Resistance Gene to Nematode Meloidogyne javanica
09 January 2024 197 1 View
Looking at the challenges faced by educators when teaching 19th century novels in a contemporary classroom.
08 January 2024 6,603 1 View
If some knows this let explain in a brief way! Thanks a lot.
08 January 2024 6,356 3 View
Now we are working on extracting large-sized plasmids(55kbp,70kbp,100kbp) from E.coli EPI300 by traditional DNA ethanol precipitation method without the spin column. But we can't get good results...
08 January 2024 1,081 5 View
Hello, I am a PhD student and I want to work on the effect of vegetation patch on river flow. I have written the proposal, but my supervisor believes that I should provide a practical...
08 January 2024 3,716 6 View
How much microplastics could be removed from wastewater using sand filtration method?
08 January 2024 5,616 3 View
I'm looking for some simple molecular biology lab experiments for under-graduates. (All I have in the lab is light microscopes, micropipettes, aerobic incubator, spectrophotometer, and other...
07 January 2024 3,597 5 View
I want to calculate dG binding energy of a protein-protein complex binding site. I choose the antibody as a receptor and the antigen as a ligand. Also I selected 10A distance from ligand for...
07 January 2024 7,210 0 View
Haarmann, H. (1990) Language planning in the light of a general theory of language: a methodological framework. International Journal of the Sociology 86, 103-26
07 January 2024 7,999 1 View
Please assist me. I greatly appreciate your thoughts, and they may be useful for my upcoming research.Thank you so much
07 January 2024 9,412 5 View
The knee assistive system has been developed based on the pattern of walking in a healthy individual using fluidic muscles. We have aimed to utilize this pattern to enhance the functionality for...
06 January 2024 1,683 0 View
I borrowed a bottle of HEK293T cell culture from someone else, but after one passage, the cell morphology became very strange and not round at all. May I ask why? We used T25 culture flasks and...
06 January 2024 5,928 4 View
Is it also possible to add iron oxide nanoparticles into the mixture during ultrasonication? Will that achieve incorporation and formation of carbon-iron oxide nanocomposite?
06 January 2024 2,106 2 View
Dear researchers, We are struggling with neverending mold contamination in all of our CO2 incubators. In the past, we used to do H2O2 decontamination/24hr-UV mode every two months now we do it...
05 January 2024 3,727 4 View
Wondering if anyone is aware of a product and/or modified protocol to randomly amplify viral RNA to give long (>1kb) cDNA fragments. I use RT-PCR with random hex to give 300-500bp, which works...
05 January 2024 8,979 0 View