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Questions related to Primer
I have developed this multiplex PCR panel for next generation sequencing library prepration. This panel is used for the diagnosis of a particular bacteria infection, as well as some SNP I'm...
26 May 2023 2,730 5 View
Los fagos serán utilizados contra Klebsiella sp.
26 May 2023 8,669 3 View
So we have to do process the samples for chip seq and these are the cells of PANC - 1. My colleague fixated these cells 2 months back and we were practicing for running the samples. So we...
26 May 2023 3,496 5 View
I am making a slurry (using mortar and pestle) of AC, AB and PVDF in the mass ratio of 8:1:1. I paste the slurry on a pre-treated nickel foam by putting the nickel foam in the mortar and then...
26 May 2023 5,059 4 View
I am performing qPCR on drosophila mtDNA. Because of the nature of my work, I use two separate primer sets. With one of them, the qPCR works as expected - good amplification with mtDNA and...
25 May 2023 8,630 3 View
I want to do virome analysis (including DNA and RNA viruses) from various tissues and organs. What would be the best approach? Which kit would be best for nucleic acid extraction and is there a...
25 May 2023 8,136 3 View
From the link https://gtexportal.org/home/datasets, under V7, I'm trying to do R/Python analyses on the Gene TPM and Transcript TPM files. But in these files (and to open them I had to use...
24 May 2023 8,778 3 View
I would like to use puromycin selection in mouse primary cortical neurons to select for transduced neurons. Have anyone had any success doing it?
24 May 2023 4,231 6 View
Hi everyone! Dose any one knows how I could get rid of the cells fluorescent background? I am working with two cells, HEK293 and SHSY5Y, and transfected them with exosome containing a...
24 May 2023 5,367 1 View
I have recently been conducting experiments with the expression of several fluorescent RNA aptamers using E. coli (BL21). However, when I induced the expression using IPTG, the bacterial solution...
24 May 2023 5,823 5 View
During a green synthesis of Copper oxide nanoparticles using a given plant extract, Nano Copper hydroxide sulfate and nano copper oxide were formed . the xrd showed the presence of both( cuo and...
24 May 2023 9,966 2 View
I have an antibody that I want to buffer exchange into PBS, as the current buffer contains high concentrations of a carrier protein. I do not know the concentration of the antibody, but that the...
24 May 2023 1,052 3 View
I have formalin-fixed paraffin-embedded samples of breast cancer and I want to extract RNA from them. Can someone recommended a kit and a protocol for that? Thanks
24 May 2023 7,414 6 View
i need help to find the Exact values of Conductivity and Permittivity of lung layers (skin fat muscel lung ) used in scientific articles for lung layers for the detection of covid-19 in the...
24 May 2023 7,803 0 View
Our group has been having difficulties finding a consistent mechanism by which to screen Bt isolates for Cry or Cyt toxin production in a quantitative, reasonably high-throughput manner. Does...
22 May 2023 270 0 View
I need to inject an antibody into the mouse's brain and record synaptic currents from brain slices 6 days after the injection. Is there any innocuous fluorescent molecule I could include in the...
22 May 2023 3,487 3 View
Long ago, I began using the program DNAStrider for my subcloning sequence analysis because it was simple, free, and did almost everything I wanted and not much that I didn't want. When Strider no...
22 May 2023 1,741 0 View
I am working in the field of forest ecology by using statistical modelling. Please write me for any further clarification. Thanks
22 May 2023 6,403 4 View
I have data for 5 sets of 10 replicates including Negative control. Using Graphpad PRISM, how to get the Pearson r and P value?
22 May 2023 9,154 1 View
We are conducting flow cytometry to detect IL-10 secretion in B cells, specifically regulatory B cells (Bregs). For this purpose, we utilize either whole blood (100 μl) or isolated PBMCs (1...
21 May 2023 2,187 1 View
In Design Expert software old versions, possibly we can have 3 to 5 levels. but the latest version is not having a feature of more than 3 levels. Please drop your suggestions..
21 May 2023 8,874 6 View
Hi all, I wonder that isolated mammalian total RNA samples can be shipped overseas (for sequencing service) at room temperature? I had a quick look and find a product via...
19 May 2023 1,659 2 View
Hi all, Just wondering if anyone has experienced similiar issues when running SDS-PAGE gels? The ladder stops running straight toward to bottom of the gel? Ponceau staining of the nitrocellulose...
19 May 2023 9,075 4 View
Hey, i've been doing a western blot like from 3 weeks ago, and i can't get good results, i'm getting this type of runs, and i don't know what can it be, maybe degraded proteins?. I'll be very...
19 May 2023 6,551 5 View