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Questions related to Primer
we want to apply some chemical component on Rats to change their Mt DNA copy number of salivary gland. is there any specific part of their mt sequence or gene we should check for this matter? is...
28 January 2024 5,663 0 View
Name of oil fields south of Iraq.
27 January 2024 4,854 1 View
For example, if I have PFAS non-target screening data for a certain sample, how can I use this data to identify degradation pathways for certain PFAS?
27 January 2024 5,453 0 View
With climate change, high temperatures, and scarcity of rain in Iraq, we find it difficult to determine future agricultural plans based on the abundance of water and temperatures and providing...
26 January 2024 7,459 3 View
We have expressed and purified the recombinant viral protein found in the insoluble fraction. Please suggest some methods or reagents to solubilize the protein.
24 January 2024 9,368 5 View
many many thanks
24 January 2024 6,789 5 View
sample preparation for penetratin (RQIKIWFQNRRMKWKK) by SPE before analyzing on LC-MS/MS?
24 January 2024 1,973 1 View
I am working on overlapping PCR with Left, EGFP and Right flank where same procedure for the primer design of the Left, Right and EGFP was using. But there is Amplification of the Left and EGFP...
23 January 2024 8,025 1 View
method of the assessment
23 January 2024 9,741 0 View
ايضاح دور الاقتصاد الدائري في إعادة السياسات الحكومية والمالية والاقتصادية إلى مسارها الداعم للبيئة ؟
23 January 2024 6,820 5 View
Green houses
21 January 2024 8,231 1 View
I've just defrosted some J774A for the first time. On thawing, the cells are small, round and not at all adherent... Is this normal? Do I need to add collagen to make them adhere to the support...
19 January 2024 259 1 View
I have got my DESeq results back - heatmaps of top 30 genes for each comparison. - I have got top 30 genes from comparison group I, these 30 are highly expressed in condition A but not in...
19 January 2024 2,092 0 View
I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...
19 January 2024 999 1 View
I have performed RFLP for WT and mutant sample. The WT sample was only PCR product, while mutant band was extracted from agarose gel electrophoresis (it was checked on gel again to see the...
18 January 2024 6,462 4 View
I'm doing Buchwald Hartwig amination and as a catalyst can I use palladium chloride instead of palladium acetate? What can be the possible issues that can come up for this change?
18 January 2024 2,121 4 View
I am using Schrodinger 2022-4 version. While I run, I get this message. "Logs of component Prime calculations suppressed. (see -report_prime_log option) " what is the reason for this message and...
18 January 2024 7,997 0 View
Soils that are physically destroyed by bombardment with various types of weapons lose their identity due to the destruction of their genetic profile. Is there any practice of rehabilitating such...
17 January 2024 2,961 1 View
Hi, I'm in the process of developing a hydrolysis probe qPCR assay to quantify gene copy numbers of Microcystis 16S rDNA. In troubleshooting my cycling parameters, I'm getting resulting plots such...
16 January 2024 6,999 1 View
hi all I need any data about the influence of avian influenza H9 on broiler and layer performance, I mean the infunece on egg production, immune response, body weight gain, feed conversion rate,...
14 January 2024 4,417 0 View
Dear Sir and Ma'am, We faced a problem in the industry that couldn't be solved despite running several tests. "When using chicken thighs for making sausages, we noticed weak tissue in certain...
12 January 2024 4,477 0 View
microbial communities
12 January 2024 8,136 0 View
tissue is human fetal cerebellum in their gestational ages
11 January 2024 4,910 1 View
i want to find the virulence factor and common gene both raw data is present here plz f some one already done plz help me
11 January 2024 5,788 0 View