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Questions related to Primer
I am trying to access my saved articles.
14 September 2025 9,987 1 View
I am looking for user-friendly tools or software that can create class schedules automatically if we provide the necessary information. Preparing a timetable for a whole department or university...
13 September 2025 4,997 2 View
To what extent does long-term compost application improve soil organic carbon and microbial activity, and how does this affect the yield stability of cereals, pulses, and oilseeds in Eastern U.P.?
08 September 2025 7,690 3 View
I'm working on a topic: Stigmatization and coping strategies as predictors of relapse among psychiatric patients: the moderating role of social support.
05 September 2025 4,264 0 View
I am performing HDV ribozyme cleavage of my RNA due to which I have to dephosphorylate the 3' end. After gel elution of my band of interest and RNA precipitation, I have 350 ug of RNA which is...
28 August 2025 3,565 0 View
How does nanotechnology enhance targeted drug delivery systems?
26 August 2025 4,563 7 View
Is it time to move beyond the conventional pollution indices (Igeo and EF), devised decades ago, and acknowledge that their reliance on fixed assumptions may render them obsolete in the age of...
26 August 2025 3,809 5 View
If I want to administer 10 mg/kg of PKM2 inhibitor (HY-103617) via tail vein injection every 3 days for 8 weeks (12 animals per group, ~500 g each), which solvent formulation would be most suitable?
20 August 2025 2,565 2 View
I am planning a systematic review and meta-analysis focusing on Toxoplasma gondii. I am looking for collaborators with experience in one or more of the following areas: Literature search and...
15 August 2025 9,221 5 View
Surveys have revealed that 75% of men ejaculate within 2 minutes of penetration. (Anthony Mason)
09 August 2025 2,657 2 View
What is the detection principle? In a protease activity assay, how can I determine whether the background fluorescence of the Gly-Pro-AMC substrate is significant?
08 August 2025 10,098 1 View
I have tried, without success, to post a summary of the life of James E. Loder who taught at Princeton Theological Seminary. Gemini (a form of Artificial Intelligence) generated the summary. I...
06 August 2025 9,443 1 View
What are the differences between Bradford (HY-K2001, MCE) protein quantification and BCA quantification?
05 August 2025 2,796 3 View
I am trying to culture liver organoids by encapsulating mouse primary cells in a photocrosslinked polymer-based hydrogel. Even with sufficient RGD and optimized medium composition, the cells only...
04 August 2025 1,124 8 View
What operational parameters (e.g., moderator temperature, neutron flux spectrum) most significantly impact weapons-grade plutonium (WGPu) purity (²⁴⁰Pu
31 July 2025 9,324 0 View
This question is very confusing for me; sometimes in some papers I note that the researcher uses downregulation of a certain protein to indicate its inhibitions.
29 July 2025 4,476 0 View
I did a western blot to detect my target protein (42 kD - 6th ladder region) then, after removing the ultra ECL off by doing a few quick rinses, I incubated my membrane with a-tubulin (5th ladder...
25 July 2025 7,868 3 View
Provide details of link to download
24 July 2025 1,102 2 View
My cells are primary human aortic valve cells, treated with ATF3 Inducer 1 (CAS No.: 3038756-30-5). Culture conditions: cultured in DMEM high glucose medium and osteogenic medium (OM) for two...
24 July 2025 9,178 1 View
I am currently working on metal–natural compound complexes. My workflow involves: Docking metal ions with natural compounds to form metal oxide–ligand complexes. Docking these metal complexes with...
24 July 2025 6,387 1 View
I’m expressing the N- and P-domains of a membrane protein (only the cytosolic regions) using a FLAG-tagged construct in the pET28b vector, transformed into BL21(DE3) cells. The cultures were grown...
22 July 2025 4,567 0 View
Hi all, I’m working on a metagenomics project focused on extracting the whole microbiome from seeds. My protocol for the DNA extraction involves soaking seeds in 0.85% saline for 4 hours, then...
21 July 2025 1,834 3 View
We tried to coat our slides with this type of agarose but it does not adhere.
11 July 2025 469 5 View
How to purify stat 1 protein if it has a his tag in ecoli bacterial cell. I am facing difficulty to purify it.
10 July 2025 3,144 3 View