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These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...
07 June 2024 2,190 3 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
06 June 2024 523 2 View
I am a master student and writing research paper. I have 3 yes/no questions, 2 multiple responses questions and 1 Likert scale question. I would like find out whether age has relationship to my...
06 June 2024 1,460 9 View
1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.
06 June 2024 4,763 1 View
microbial contamination of food
06 June 2024 7,581 1 View
I had a valid idea for a meta-analysis involving multiple prospective & retrospective studies with no prior meta-analyses on the same chosen sub-population. When confirming the validity of my...
05 June 2024 8,592 1 View
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
04 June 2024 2,779 7 View
Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...
04 June 2024 7,614 6 View
Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....
04 June 2024 4,051 3 View
I received it from: [email protected], with the following text. Dear Author, Welcome! Your recent publication has been provisionally selected for the "Best Researcher Award."...
03 June 2024 3,629 2 View
Ethnobiology
03 June 2024 444 6 View
The concept of "creativity" in a literary text, excusing poetic texts, refers to the ability of the author to produce work that is original, imaginative, and expressive. Creativity in literature...
01 June 2024 3,953 3 View
I know that we can categorize a specific linguistic change as either gramaticalization or subjectification (or both involved), but can we also say that specific linguistic change is motivated by...
01 June 2024 2,010 2 View
I am preparing RNA later following the patented protocol. It is mentioned there and in other recipes as well to use H2SO4 to balance the pH to 5.2. If H2SO4 is not available, can I use HCL instead...
31 May 2024 3,069 0 View
It’s important to see three points: (1) change is possible; (2) it isn’t his fault that he is the way he is; and (3) there is no better alternative. (Kramer & Dunaway)
30 May 2024 6,235 0 View
Pl. share insights into T-cell isolations optimizations/criteria and stimulation agent quantities required and incubation times optimization for check point inhibitors in vitro assays development....
30 May 2024 4,577 0 View
Hi, I was wondering if anyone using the TWIST NGS Methylation protocol has experienced issues with under-conversion of CpGs? Thanks.
30 May 2024 4,664 0 View
Hello, I have currently been tasked with performing a split GFP analysis. I have been using DH5a cells for plasmid transformation. The results were turning out to be what we expected. The...
30 May 2024 5,694 0 View
My vector is 7000 bps, and my insert is 1300 bps
29 May 2024 736 4 View
Hi, I need help. I am conducting a systematic review of quantitative research. However, several of the studies I have included are MMR, and I want to assess the quality of those articles using...
29 May 2024 4,072 3 View
How do I edit the list of papers I incorrecty entered as an article author pls
29 May 2024 5,131 1 View
What is the role of crop diversification in sustainable agriculture and how crop diversification can benefit farmers, consumers and the environment?
27 May 2024 2,858 8 View
according to your academic experience the immune system will overcome the cancer challenge in future .
27 May 2024 2,687 3 View
What is the importance of biodiversity in relation to sustainability and difference between diversification of crop production and diversification of productive activities?
26 May 2024 5,658 11 View