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Questions related to Primer
I want to do the genotyping analysis.
08 August 2023 9,164 1 View
I intend to perform cytokine measurements in rodent samples and would like to use the bead based cytokine multiplexing analysis, preferably using the Bio-PlexTM Pro Multiplex Immunoassays. If...
07 August 2023 4,069 2 View
Prof. Van der Wolf with colleagues (2005)* wrote that for "... Clavibacter michiganensis no data on transmission via nematodes are available". Dr. Osdaghi (2022)** in the most recent review of...
05 August 2023 7,752 3 View
I did a transformation of the BL 21 cells with my plasmid DNA. Then I ran the dna plasmid pcr to check the inserted dna and both samples showed bright, clear bands. However, when PCR with a larger...
04 August 2023 6,437 2 View
Is it possible to grow green manure without soil disturbance in zero till system and what is permissible limit of soil loss in India?
04 August 2023 2,519 3 View
What's your experience with this conjugation method? I want to try a proximity ligation assay and I know there's kits out there that seem to be quite pricey and there are methods to simply bind...
04 August 2023 2,399 3 View
So I am performing a routine site directed mutagenesis of a ~ 7kb plasmid. After PCR I DpnI digest my template DNA and then transform my PCR product into DH5a cells. I use a partially overlapping...
03 August 2023 2,586 2 View
Looking for any research papers or examples.
03 August 2023 1,609 4 View
I want to conduct click reaction with azide in Fmoc-SPPS.
03 August 2023 3,142 0 View
Good day. I am looking for expert validators to check the suitability of our questionnaire in THE IMPACT OF CUSTOMER EXPERIENCE IN SOCIAL MEDIA MARKETING ON RELATIONSHIP QUALITY TOWARDS PURCHASE...
01 August 2023 6,843 0 View
Zn-BDC and Co-BDC MOFs
01 August 2023 4,759 3 View
I ahve problem with 20ul final reaction volume. my Positive control works and i have a good amplification curve but my DNA samples do not work! is there any fixed reaction volume that has worked...
01 August 2023 8,323 4 View
Some of my data points are outside the standard deviation, so I want the error bars to capture them by doubling the SD values. Is there a way to do this? How? If not in GraphPad, how about in Excel?
01 August 2023 4,737 2 View
Hi, I am seeing cell clumps in single cell suspension of splenocytes. I use DMEM media+HEPES+Gentamycin. Then I resuspend the cells in AIM-V media. The cells get clumpy and they can even be seen...
01 August 2023 5,537 0 View
Is there a list of the spider families in which are absent cheliceral teeth and/or serrula? I know that some families lack of chericeral teeth (e.g. Thomisidae) or serrula (e.g. Zodariidae) but a...
01 August 2023 5,036 2 View
Is it possible that the amplification failure products can be visualized in electrophoresis? Due to the failed amplification results it shows bands in my electrophoresis with bands that are quite...
01 August 2023 5,703 3 View
I need to quantify images from immunofluorescence assays. Which is the minimum number of images to be taken when I take into account the reactivity area of positive cells to a specific marker? If...
31 July 2023 3,405 3 View
Transformation
31 July 2023 4,920 2 View
When I used 0.5g of air-dried soil and added 1ml of chloroform to fumigation it overnight in a fume hood, then extract with 0.5mol/L NaHCO3 within pH=8.5, there was no significant change in...
31 July 2023 6,075 5 View
I have set up a PCR using primers that amplify only the exons of my gene of interest. The template I am using is cDNA (mouse samples). I set up this PCR a year ago with an annealing temperature of...
31 July 2023 229 15 View
Leaves of several plant species lost chlorophyll after infestation by Spanish slugs (Arion vulgaris). Is it specific slug toxin or some specific virus vectored by the slug?
30 July 2023 2,179 5 View
I am looking for advice from experienced individuals regarding the optimal conditions for plasmid electroporation in A375 cells. Would anyone be willing to share their expertise on this...
30 July 2023 3,444 4 View
Does anyone (in the world) has the book of the 8th Symposium on Bacterial Genetics and Ecology (BAGECO 8), that happened in June 26-29, 2005, Lyon, France? If yes, can someone send a copy of...
30 July 2023 8,381 0 View
I am doing my master thesis and Need emergency help
26 July 2023 9,889 0 View