Dear Feba Anna John ,
Even though I do not fully understand your problem, I can provide you with some comments that hopefully are useful:
First, you need to know if the 1 mg/mL is related to 1 mg of total mass (nanomaterial + solvent) or only nanomaterial, pero mL of total solution. This will influence the total mass of your nanomaterial (or active principle) that you will have in 500 microliters. In 500 microliters (0.5 mL) you could either have 0.5 mg of total mass (nanomaterial + solvent) or 0.5 mg of only nanomaterial. This will depend on how the solution was prepared.
Normally, to "load" 500 microliters, you could use an automatic pipette, that allows you to choose the exact volume you want to work with.
If you deposit this volume in a filter paper disc, this will absorb in the disc completely I believe. Just keep in mind that you should probably deposit the solution drop by drop, in different parts of the paper disc, just to avoid dripping.
Faite des dilutions puis impregnè le disque de papier buvard. Déposer le disque a la surface du milieu prè-ensemencè.
Incuber et mesure le diamètre. Comparer au normes de Vincent.
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