Primer

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Questions related to Primer

Is the CD1 in QIAGEN DNeasy PowerSoil Pro kit same as the CD1 in QIAGEN DNeasy Plant Pro Kit?

Is the CD1 buffer of QIAGEN a sodium thiocynate-based lysis buffer? Does it have any other detergents? Does anybody have the composition of CD1 buffer?

29 October 2024 3,928 0 View

Why are men sexually proactive and women sexually passive?

Heterosexual contacts occur more frequently because they are facilitated by […] the greater submissiveness of the female and the greater aggressiveness of the male, and this seems to be a prime...

29 October 2024 2,973 0 View

RNAlater on non-anticoagulated blood samples?

Hello, please i would like to know if I can add RNAlater to non-anticoagulated blood sample in order to stabilize and protect RNA. If yes, which volume to add ?

29 October 2024 5,606 0 View

How I can get GFRP material properties for sikawrap 430G for modelling in abaqus?

Plz explain

29 October 2024 6,070 0 View

Does anyone have a copy of the SCORS (reference below)?

Westen, D., Lohr, N., Silk, K., Kerber, K., & Goodrich, S. (1985). Social Cognition and Object Relations Scale (SCORS): Manual for coding TAT data. Unpublished manuscript, University of...

28 October 2024 7,495 0 View

What steps should I follow to wrap GFRP around a circular column?

When I have limited GFRP material properties, Are there any typical values or industry standards I can rely on for modeling the GFRP wrap in this case?

24 October 2024 780 2 View

Frozen blood- RNA ISOALTION TIP?

We are currently facing challenges in isolating RNA from frozen blood samples. Despite utilizing RNA isolation kits and following the TRIZOL protocol, our attempts have consistently yielded no...

23 October 2024 4,735 3 View

Why I m getting brittle failure in concrete column subjected to 4mm deflection?

I am modelling a UHPFRC jacketed column in abaqus. After reaching ultimate load sudden decrease in load is observed...ACC to experiment it should be gradual decrease.

22 October 2024 2,071 4 View

How to overcome a hairpin problem while performing in vitro transcription?

Dear all, I am performing an in vitro transcription of 300 bp long ALU RNA, 600 bp long ALU RNA (two copies) and 900 bp long ALU RNA (3 copies). While 300 and 600 bp templates seamed to provide...

21 October 2024 4,181 0 View

Etica para el uso responsable de la IA?

etica para el uso responsable de la IA

21 October 2024 5,445 0 View

What is your job title?

implementation professional

20 October 2024 8,910 2 View

Isolation of neutrophil RNA from whole blood - how to stabilize RNA?

Dear all, I am planning to isolate neutrophils from whole blood of pigs for later RNAseq analysis. The samples may be taken over the course of several hours, so I assume I will have to stabilize...

18 October 2024 3,528 1 View

Should my co-supervisor who did not contribute to the writing of my article (article was based on my thesis) be included as co-authors in my articles?

Hi I completed my thesis and would like to publish articles based on my thesis studies. For one of my articles, that is based on a study that I conducted during my thesis, only my main supervisor...

17 October 2024 5,089 7 View

What could be the reason of very low cluster passing filter for my Aptamer pool Sequencing in MiSeq?

I have very low cluster passing filter for my SELEX pool Sequencing in Miseq using Nextera Miseq reagent kit V2 (300 cycles) and XT index kit set B. I performed all QC of pools after Adaptor and...

16 October 2024 4,521 0 View

When analyzing qPCR (SYBR green) results, how can I discriminate between absence of target gene or low presence when the Ct are high (33-36)?

I'm trying to detecte very small amounts of a mutant-type mtDNA using SYBR green qPCR. I can't really use a higher concentration of gDNA because my wild-type and housekeeping genes already have a...

15 October 2024 1,476 3 View

Can anyone recommend me a software to calculate heterosis?

I want to calculate heterosis. anyone know as application or online about it?

15 October 2024 3,501 3 View

In geologic studies, why do oxygen fugacity calculations focus on only a few minerals in a rock?

Dear Professor: I hope this email finds you well. I am currently facing a question regarding the calculation of oxygen fugacity, particularly about the specific minerals chosen for these...

11 October 2024 7,942 0 View

What is the best Fe Skarn mine in world?

I want to know more about Fe skarns in world.

11 October 2024 5,276 1 View

Are There Publication About Citrus Juice Processing with Vacuum Evaporatif ?

Vacuum Evaporatif Machine or aparatus to held Citrus Juice processing,

10 October 2024 168 1 View

Cell transplant in unilateral and bilateral PD model monkey selection consideration?

Any infection on pharmacodynamic evaluation results？

09 October 2024 1,657 0 View

In your opinion, does the use of virtual labs help engineering students carry out practicals and achieve practical objectives?

Virtual lab helps lecturers a lot in making it easier for students to understand basic practical concepts in a topic. But is this method really able to help achieve the practical objective?

09 October 2024 7,435 2 View

How to measure light intensity delivered to my solution inside a curved, double-walled glass reactor?

I would like to measure the light intensity delivered to my solution inside the double-walled glass reactor, as shown in the attached picture. I am using a Thorlabs PM100A power meter, which...

08 October 2024 3,702 1 View

Ethical and Fairness Issues in Artificial Intelligence?

1. how to solve the problem of algorithmic bias in AI? It seems to me that the datasets used by AI often contain a historical bias that affects the fairness of decisions. I would like to know what...

08 October 2024 2,678 1 View

What impact will inflation have on the current world economy and what changes will it bring to the subsequent economic trends in the United States?

According to the current economic trend, I want to know more about the impact and changes that inflation has brought to the current market. Maybe it is life, work, economy, investment, funds,...

08 October 2024 3,656 0 View

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