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Questions related to Primer
I work with large proteins and try to transfer my western blot studies for his reason ı using 6% gel. In a semi-dry transfer system, my all proteins are transferred in 25V 1.5amper 9 minutes ı...
10 June 2023 794 2 View
I am new to the field of exosome research and I have isolated exosomes from BMSCs. However, I have noticed that these exosomes do not show efficient effects on OA chondrocytes. I would like to ask...
09 June 2023 8,791 3 View
The 1956 paper A simple ultraviolet spectrophotometric method for the determination of protein is constantly referenced as the one who provided a method for peptide concentration calculation that...
09 June 2023 922 2 View
I’ve queries regarding cryopreservation of mammalian cells in DMSO the queries are as fallows: Q) How DMSO will protect cells from intracellular ice formation during freezing? What I know is It...
09 June 2023 108 2 View
What could be the possible reason for not getting proper band of ladder while performing PAGE
08 June 2023 7,636 1 View
Why is melting temperature important in Primer Design?
08 June 2023 1,741 2 View
Hi! I'm working with MSC from Umbilical Cord for exosome isolation. I used beta amyloid to induce/increase the exosome production and normal culture conditions to compare. I'm using UC protocol:...
08 June 2023 5,744 2 View
I am attempting to amplify a small amount of genomic DNA into a much higher quantity for future experiments using random hexamers (NNN NNN) as the primer set. Currently, I am running this protocol...
08 June 2023 2,740 1 View
In my experiment, I utilized a combination of Poloxamer 188 (Kolliphor) and Gibco Anticlumping agent in Dynamis media at a concentration of 0.1% per liter for CHO S cell culture. Surprisingly, I...
08 June 2023 7,470 1 View
I have prepared 12% of SDS gel and a sample concentration that needs to load 24 ul in each lane to fulfill the requirement of 4uM protein concentration and the maximum volume of the lane is 20 ul....
07 June 2023 4,728 1 View
When designing a Taqman probe in real time PCR, what are the factors that affect the signal intensity? I mean I know mismatches decrease the intensity and a probe shouldn't start with a G, but...
07 June 2023 8,720 2 View
I want to integrate 5 GEO datasets and analyze them together. However, I don't want to include all the samples in all the GEO datasets but to include a specific phenotype along with the control...
07 June 2023 2,205 1 View
I have expressed my protein of interest in E.coli and purified it via the Ni-NTA column. Then did a UV-VIS spectroscopy to see if it is binding to its chromophore. Even after several trials, it...
07 June 2023 4,364 6 View
I work with diploid and sexual species. Currently, I have AFLP data from populations of a self-sterile species and a self-fertile species. I was asked to estimate observed (Ho) and expected...
06 June 2023 9,342 2 View
Non fibrillar collagens are mainly seen in basement membrane but I would like to know if they are produced by osteoblasts or osteocytes also
06 June 2023 9,506 1 View
I want to assess Chloroquine and Primaquine Treatment Outcome among Uncomplicated P.Vivax Malaria: Open Label Clinical Trial.Which Study design is suitable for me?
06 June 2023 9,574 6 View
Hello Everyone I have overexpressed one gene in cardiac cells. I notice so MVBs but to confirm that those are MVBs, please can anyone suggest me different markers which I can used to do further my...
06 June 2023 4,931 3 View
When i set up my culture from a plate and do a spot test to check the presence of phages, the culture is not able to grow in the plate and I am unale to see the spots. The culture reaches log...
06 June 2023 9,725 2 View
Hello everybody! The amidation of 4-dodecyloxybenzoic acid methyl ester doesn't work at all according to the publication "Perylene liquid crystals with multiple alkyl chains: investigation of the...
05 June 2023 7,998 7 View
Hello, I have multiple pathways that are being studied due to single-cell sequencing. What is the best way to represent pathways or specifically KEGG pathways for a manuscript? Other than...
05 June 2023 3,954 3 View
I've done 2ns protein-ligand MD simulation in two ways 1. first I used general MD simulation, generating every single file by commands as suggested in gromacs tutorial. As an output, I've got 200...
05 June 2023 9,397 4 View
Good morning to all if i have following random DNA sequence then how to find intron and exon manually for Hidden Markov model...
04 June 2023 5,442 2 View
"RTMS induces changes in FDG brain metabolism in patients with epilepsy" by Laura-Seynaeve
03 June 2023 6,108 4 View
I have overexpressed one gene, and I noticed that whole MVBs are releasing out, instead of forming the bud with the plasma membrane and releasing the stored material. Could someone suggest to me...
03 June 2023 207 0 View