Primer

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Questions related to Primer

Can plants interact with soil microorganisms & contribute to soil fertility &role of microorganisms in decomposition of organic matterpresent in soil?

How do plants interact with soil microorganisms and contribute to soil fertility and role of microorganisms in the decomposition of organic matter present in soil?

09 March 2024 2,804 5 View

When you guys want to deplete bmp4, you usually do not use bmp4 gene knockdown directly?

I recently start and try to research the Bone morphogenetic protein 4 (bmp4) gene. I want to inhibit BMP4 gene expression and so I think bmp4 gene knockdown using si or shRNA is okay. but when I...

09 March 2024 5,142 0 View

I really need your help regarding paper placement. Ideas?

I have an unusual problem in deciding how to place this article I wrote with two colleagues. I am a psychological trauma expert, but also a statistician. During COVID, as my students turned to...

08 March 2024 8,947 3 View

Hello, How can i make a primer that amplify a same gene (CRa) in three different plant species?

Actually, I am trying for months to make a primer of a Clubroot resistance gene (CRa). CRa gene is found in three Brassica species. My aim is to make a primer that can amplify this one gene in all...

07 March 2024 9,832 4 View

Partial pressure Order calculation?

Hello, what is the best way to find partial pressure order. I am proposing the following and correct me if I am wrong ? Obtain Activation Energy and pre-exponential factor from ( Coat-redfern)...

07 March 2024 1,022 2 View

What's effect of crop residue management on soil properties & impact of crop residues burning on soil fertility & agriculturally important microbes?

What is the effect of crop residue management on soil properties and impact of crop residues burning on soil fertility and agriculturally important microbes?

07 March 2024 1,990 0 View

Why do you use caco-2 and ht-29 cell lines as healthy cell lines to evaluate the effects of drugs and commercial foods?

Thanks

05 March 2024 1,462 0 View

How does Sysmex DI 60 measure the reliability in evaluating RBC, WBC, and Hands-on time analysis? What are the inconsistent results detected?

This question is based on this article: Red and white blood cell morphology characterization and han...

05 March 2024 685 3 View

Could anyone provide guidance on the reliability and accuracy of docking scores obtained from PyRx and SeeSAR software tools?

Dear all, I am currently conducting a research project involving protein-ligand docking, and I have encountered discrepancies in the results obtained from two different docking software tools,...

03 March 2024 4,157 0 View

Is it possible to transfer blood from animals to humans?

Is it possible to transfer blood from animals to humans?.

02 March 2024 5,689 3 View

Would anyone could tell the reaction kenetic energy between ZnO and Si?

We deposited ZnO on Si, and observed a thikening of interface SiOx when annealing morethan 500℃. I'd like to analize why this temperature is specific, I don't know how to describle it.

02 March 2024 6,351 2 View

How do I calculate the correct primer concentration or molarity?

I was told that I have to post concentrations of primers in µM per amplified DNA sample in the documentation of a qPCR instead of absolute volumes. However, I am not sure how to do that. I used...

01 March 2024 4,447 2 View

Omasal sampling technique and omasal digesta reconstitution?

Hello, Does anyone have experience with omasal sampling technique using double-marker ruminal infusion system (Cr-EDTA and YbCl3)? I have fractioned my pooled omasal digesta in to three phases:...

01 March 2024 6,550 0 View

Why were the cells observed to be dead in the scratch assay?

I used a 12-well plate, and there were 1.5x10^5 NIH-3T3 cells in each well. After 20 hours, when I used a pipette tip to create a scratch and changed the medium, a portion of cells at the well...

27 February 2024 4,858 0 View

Options for nonparametric alternatives to a T test?

I have data extracted from literature on biomass production and related variables. I would like, if possible to conduct a seried of factor based analyses comparing biomass growth on managed and...

27 February 2024 1,685 1 View

Which peptide will be translated in this situation?

I recently encountered an intriguing situation while examining a plasmid constructed by someone else for a eukaryotic expression system. This plasmid contains a unique arrangement of open reading...

27 February 2024 2,290 2 View

What factors cause the increase in the average number of employees being rehabilitated?

This is about work rehabilitation program

24 February 2024 4,069 0 View

Can anyone recommend a software that is suitable for a nonlinear polynomial regression and splines or example on how to use it?

I am conducting a meta-analysis and I want to use the nonlinear polynomial regression and splines functions to model the dose-response relationship between the parameters of interest. I would...

24 February 2024 835 3 View

Can Flavonoids and phenols be found in higher concentration in Non-polar solvents in case of millets?

How to identify the phytochemical compounds.

23 February 2024 4,343 1 View

IPSC cells not growing after thaw, have tried all sorts of troubleshooting??

Hello Everyone, I am having some issues with regards to some iPSC stocks I have been trying to revive for a while. The initial lines that we ordered thawed fine and grew fine, so we froze down a...

23 February 2024 4,967 1 View

Does anyone have stimuly for Stroop taks with numbers or arrows and can share them?

I'm interested in tasks which feature numbers and arrows as stimuli that are implemented in classical Stroop paradigm. Specifcally, number version of this task feature numbers of different sizes...

22 February 2024 122 0 View

What types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans?

I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...

22 February 2024 7,081 1 View

Can I freeze the protein samples with laemmli at -20ºC and then denature them before running the gel in a western blot?

At the protocol I'm using now there is an added note indicating that it is possible to freeze the protein samples mixed with the laemmli to temporarily store them at -20ºC and denature them before...

21 February 2024 8,199 2 View

The pH of the deionized water in the lab is suddenly much higher. Any thoughts why?

One day when we made our regularly used medium, we noticed the initial pH was much higher than usual (above 6.0. usually it is around 4.0). It seems it only happened in our lab. The neighbor lab...

20 February 2024 5,199 4 View

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