Primer

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Questions related to Primer

How to seed cells for extended siRNA experiments?

I am currently conducting experiments trying to do some protein knockdown using siRNA. I have read that full mRNA knockdown occurs around 48 h after siRNA treatment, but protein depletion occurs...

26 July 2023 3,544 4 View

Why my EAE induction is not working?

Hi everyone, I am unable to understand that why my EAE induction is not working. I have been using MOG35-55 (50ug/mice) in CFA emulsion. Pertusis toxin 200ng/100ul per mouse on Day 0 and Day 2 of...

25 July 2023 2,354 2 View

How to view the fastq files in the Linux system?

I have downloaded fastq files but when I open it black screen appears. Is there any specific software or command for viewing the fastq files? I think it is opening in the text editor in Linux system.

25 July 2023 9,999 2 View

Why is it that the Zebrafish embryos are not surviving?

I am working on developmental toxicity in zebrafish embryos. I have optimized and completed treatment for various toxicity assays. Now I am not able to maintain embryos till the required time (3...

25 July 2023 274 0 View

E coli total RNA on PAGE gel - what are these bands ?

I run E coli total RNA on 10% TBE-Urea PAGE denaturing gel, and just wanted to know what RNA species these bands exactly are. I used an RNA ladder contains ssRNA of 1000, 800, 600, 400, 300, 200...

25 July 2023 4,326 3 View

How to model a truncated protein?

Hello, I am working on modelling a truncated protein (E520* for example) due to a stop-gain mutation. I am trying to show how the protein structure changes and I need to calculate back-bone...

24 July 2023 1,679 0 View

Is there a way to remove Heparin from blood sample?

Hello, I have blood samples that were collected in heparin. The end goal is to work with the plasma, but I need to first remove the heparin from the blood. Any suggestions on how to...

24 July 2023 8,908 3 View

Discrepancy between Linf and Lmax estimates in ELEFAN_GA analysis using TropFishR: How to Resolve?

Dear ResearchGate community, I am currently using the ELEFAN_GA function in TropFishR to analyze monthly length frequency data and estimate growth parameters in fisheries biology. During the...

24 July 2023 7,603 2 View

Is Potato Dextrose Agar (PDA) medium suitable for bacterial growth ?

Potato dextrose agar often abbreviated as PDA. Most mycologists widely use it as a general-purpose medium for yeasts and molds that can be identified to some extent based on their morphological...

23 July 2023 5,744 22 View

Could ionomycin or A23187 bring calcium ions into cytosol from culture medium?

We knew that traditional DMEM contained several mM calcium ions while cells' cytosol are nM range. Therefor, it seemed to me that A23187 or ionomycin could bring a huge amount of calcium ions from...

23 July 2023 580 1 View

Could I use O-nitro- phenyl-a-D-glucopyranoside or maltose as a substrate for a-glucosidase inhibitory?

Could I use O-nitro- phenyl-a-D-glucopyranoside or maltose instead p-nitrophenyl-a-D-glucopyranoside as a substrate in a-glucosidase inhibition by plant extract?

23 July 2023 1,570 2 View

Dot shape protein ladder, Is my protein ladder degrade?

Hi it's my second time running sds page. I use 90 volt, 10 ul sample and 5 ul protein ladder Thank you

23 July 2023 2,397 3 View

How to obtain the structural fragment information of matter by MSMS fragment?

I have no idea what kind of specific structure it is, which makes it is aproblem to isolate it.The m/z and MSMS are the only information we have got right now.so I want to know its structure as...

20 July 2023 7,096 1 View

After how much time, I can upload my manuscript on ResearchGate 'publicly' after publication in copyright (subscription-based) journals?

Manuscript, ResearchGate, publication, copyright journals

20 July 2023 9,385 3 View

Could someone help extend my knowledge of Molecular Dynamics?

Greetings great scholars. I am new to molecular dynamics simulation. I am studying the solvation dynamics of a terpolymer optimized in four solvents; dmso, ethanol, methanol, and water. I...

20 July 2023 3,201 2 View

Primers dimer in PCR?

Hey All, How i can avoid or prevent Primers dimer during PCR amplification? I Know about the solutions that creating optimals primers and using proper primers concentration, also to change or...

20 July 2023 4,711 6 View

Where can I find the Liz 1200size standard for Gene marker software?

Hello dear scientists Where can I find the Liz 1200 size standard for Gene marker software ?

19 July 2023 1,362 1 View

Which method better to extract the phytochemicals?

Which method better to extract the phytochemical from plant by different solvents starting from non polar solvent to high polar (Solid- Liquid Extraction) or do extraction first in 80 MeOH then...

19 July 2023 4,114 7 View

Cómo extraen RNA total desde vesículas extracelulares provenientes de suero/plasma?

Estoy teniendo problemas con la extracción de RNA total desde vesículas extracelulares (VEs) plasmáticas. Previamente traté el plasma con trombina para obtener una muestra serum-like y luego...

19 July 2023 6,006 1 View

What brand of 12-well cell culture plates can withstand a 2000g spinfection without breaking?

Several members of my lab have been attempting to perform a retrovirus or lentivirus spinfection into CD3 T cells (mouse and human). The ideal plate would be a untreated 12-well plate with the...

19 July 2023 7,171 0 View

Who are some publishers where I can write a book chapter?

Are there any publications where I can write a book chapter? My expertise is data integration and analytics. Have 15+ years experience. thank you!

19 July 2023 6,338 3 View

How could I choose the right number of cells and the right plate to perform drug test experiment?

I am working with triple negative breast cancer cell line MDA-MB-231. I want to evaluate the effects of a specific human rrecombinant protein on them and to evaluate cytokine production in...

19 July 2023 2,060 5 View

What number of intronic basepairs adjacent an exon would be important to look at for detecting issues with splicing/etc?

We are running a project where we are Sanger sequencing gDNA for a specific gene (DPYD), and doing sequencing reactions for each of the exons. The reads we get include intronic bp on each side of...

18 July 2023 4,880 2 View

Gene dosage and protein expression are linear corelated?

I am interested in the relationship between gene dosage and the amount of protein expression. Any one has experience in this concern? If there is a consensus?

18 July 2023 6,320 4 View

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