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Questions related to Primer
I have added shelf life, adjuvent too but there is no improvement in bloating of bottle
04 July 2023 6,890 1 View
In these results, the LOC presented a sequence of numbers, such as LOC102550392, LOC102550392 and LOC102550980. Thanks
02 July 2023 1,316 2 View
No
02 July 2023 7,118 5 View
I am having a hard time doing custom taqman RT-PCR genotyping because I designed my own primers and probes. I can say the protocol is working, but I am getting very low fluorescence intensity for...
01 July 2023 7,351 6 View
I'm performing qPCR on bovine whole blood samples collected 9/2021-11/2021 and stored/thawed a handful of times before initial PCR testing for anaplasmosis locally and then for BLV via PCR at...
01 July 2023 5,576 3 View
Double immunohistochemistry Topics, double immunofluorescence Topics, or papers involving.
30 June 2023 4,190 2 View
Anyone working on machine learning model or any other approach regarding this?
30 June 2023 3,518 1 View
Hi, What are some easy methods to find RNA binding proteins? Best, Priyadarshini
30 June 2023 1,868 2 View
Dear colleagues and friends I have encountered a problem with my Microcystis culture. I tried isolating its protein and centrifuging it to collect the biomass. But the biomass won't settle down...
29 June 2023 1,689 2 View
Hi, I would like to know the best method of preparing nanoparticles containing amino acids. I have tried nanoprecipitation method but the entrapment efficiency is very low.
29 June 2023 6,996 3 View
I would like to download all the gene amino acid sequences for one KEGG orthology, but continuing encountering issues during the process. I intended to download 4000 genes, but always stopped at...
29 June 2023 8,830 1 View
Hello all The Cartridge for iseq-100 illumina was thawed and the re-frezing. Illumina did not recommend this, But I do not want just to throw away the cartridge, I would like to use it. Does...
29 June 2023 2,096 0 View
I am using a PCR amplified product of around 56 bp with a T7 Promoter sequence and I am getting a single band after PCR. However, after in-vitro transcription of this PCR Product, I am getting two...
28 June 2023 6,224 4 View
Can any one help me in this regard - Molecular characterization of bifidobacterium by AFLP ? or RFLP?
27 June 2023 1,014 1 View
Hello all, I have recently started working with cytosolic/nuclear fractioning of HEK293T cells. My first few attempts were unsuccessful, in that there seemed to be pretty much equal...
26 June 2023 9,973 0 View
What is the difference between nitrogen-fixing bacteria and nitrifying bacteria and difference between nitrogen cycle and nitrogen fixation?
24 June 2023 4,405 5 View
Hello all, I am used to using the TurboBlot for Western Blot and had no issues with exception of transferring high MW proteins. That's why I wanted to try the Wet transfer method. However,...
24 June 2023 2,323 4 View
Hello, I recently received metagenomic 16S rRNA gene sequence data from a company, which includes both raw reads, and clean data with barcodes removed. My goal is to analyze these sequences and...
24 June 2023 7,052 9 View
We isolated the genomic DNA from human blood sample by agarose gel electrophoresis but didn't receive a proper band what may be the reason
23 June 2023 1,586 3 View
Using RNAscope® 2.5 HD Detection Reagent – RED on FFPE sections
23 June 2023 2,827 0 View
Writing Plagiarism Free Research, Research Language and Selection of the Words for Writing Publishable ResearchArticles
22 June 2023 7,991 2 View
I am experiencing issues with the expression of phosphorylated proteins in my western blot experiments. Specifically, I observe strong phosphorylated protein expression but no expression of the...
22 June 2023 3,685 3 View
Hello everyone, I am using the discovery studio for the visualization of the Protein-DNA interaction as the 2D plot but it shows some error the ligand fragment is not selected, how I fix this...
22 June 2023 9,367 4 View
Does anyone have experience isolating microRNA from isolated mitochondria for sequencing?
22 June 2023 3,134 0 View