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Questions related to Primer
What is the role of biogeochemical cycle in sustaining life on Earth and what biogeochemical cycle do bacteria help plants grow through?
08 May 2024 3,948 5 View
Why are microorganisms important in forming soils and recycling and role of microorganisms in soil fertility nitrogen cycle?
08 May 2024 307 3 View
hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...
06 May 2024 2,312 0 View
Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...
05 May 2024 3,571 2 View
Hello, I received some GWAS results. There is a column for SNP, position, REF/ALT allele, p value, SE, which is pretty straightforward. However, I am confused about the column titled 'Effect',...
04 May 2024 330 1 View
Greetings everyone, I am currently tasked with conducting DNA extraction from Aspergillus isolates, utilizing the QIAgen genomic buffer set kit. Regrettably, not all the components outlined in the...
04 May 2024 4,999 0 View
Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...
03 May 2024 6,336 1 View
I need clarification on the appropriate timing for administering prophylactic antibiotics to cancer patients.
30 April 2024 7,130 2 View
Hello. I have try to immobilize the thoilated aptamer onto the gold surface such as Gold coated silicon wafer and the gold coated screen printed electrode. However, it is failed, after aptamer...
30 April 2024 4,066 1 View
medical education and quality assurance are linked?
30 April 2024 1,787 2 View
Me interesa saber la importancia de esta, así como su problemática
30 April 2024 3,557 0 View
I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...
29 April 2024 2,138 3 View
how to do calibration curves for the prediction models in SPSS, i think it is important in addition to the external validation
27 April 2024 2,053 3 View
How does uneven heating of land and water on the Earth bring rainfall and unequal heating of the earth's surface cause wind and ocean currents?
26 April 2024 4,334 5 View
How Dynamic Bayes can be better integrated with cloud models for risk assessment
26 April 2024 5,646 2 View
How does the top predator affect the ecosystem and what would happen if secondary consumers were removed from an ecosystem?
25 April 2024 6,840 0 View
I seem people list their Areas of Specialization (AOS) and Areas of Competence (AOC) on their academic CV's, but it is not clear to me how these categories are distinguished from each other. Is...
25 April 2024 4,102 2 View
Next step is doing qPCR. Thanks
24 April 2024 5,459 5 View
Hey all, I was wondering if you all have an indication of a company or university lab in the USA that does antimicrobial residue testing in soil. Please, let me know! Best, Laura
24 April 2024 1,089 1 View
which regression analysis will be applied if there are 7 nominal dependent variables(YES/NO categories) and 1 ordinal independent variable(3 categories: Low, normal, high)?
24 April 2024 9,989 3 View
Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...
24 April 2024 6,655 0 View
There are many methods are available to predict soil available nutrients such as soil testing, plant testing, nutrient deficiency / toxicity symptoms on plant foliage etc. and recommending...
24 April 2024 9,506 3 View
Because so far, the petri dish we got are only treated at the bottom part of the petri dish not the lid. Can the spheroid still form?
22 April 2024 9,694 3 View
Hello, I am in charge of discovering new compound candidates using AI. Currently, we want to create hundreds of thousands of virtual compounds and then select final candidates through...
22 April 2024 9,800 0 View