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Questions related to Primer
How are equations applied to assess the risk of heavy metals in vegetables?
14 May 2025 787 3 View
I came across this article https://www.nature.com/articles/s41598-023-30774-x. I wonder if anyone ever tried for embryo transfer purpose? Thanks
14 May 2025 2,047 1 View
I am analyzing whole-exome VCF data from individuals with precocious puberty to identify novel or unique variants not previously associated with the condition.
10 May 2025 6,932 1 View
T2D is quite a complex scenario with up- and down-regulation of cytokine expressions. In case of poor controlled T2D (contribution of hyperglycemia/high blood glucose and glycated serum...
10 May 2025 6,989 4 View
Hello, I'm interested in optimizing the cost-efficiency of the purification of PCR products from macro fungi for Sanger sequencing using Exo+Sap mixture. My Exo+Sap product protocol suggests...
09 May 2025 347 4 View
Based on the phylogenetic tree attached, is Bacillus tequilensis more closely related to Bacillus safensis or Halalkalibacterium halodurans? Please let me know why too.
04 May 2025 7,100 1 View
In our study, we found that a certain transcription factor positively regulates genes involved in pullulan biosynthesis. However, when this factor is deleted, genes related to melanin biosynthesis...
02 May 2025 9,019 3 View
Has anyone seen something like this in Hoescht staining of OVCAR8? I am not sure if this is fungal contamination since it is in the cell and I see no sign of contamination in culture as the cells...
02 May 2025 8,796 1 View
I conducted kinship analysis for my Colchicum study - once within the sambaR RStudio package, and the second time by using the KING tool from the terminal, and then visualising the results in...
01 May 2025 1,712 0 View
For some reason I have a bit of a stockpile of spin columns from Qiagen plasmid miniprep kits, NEB Monarch plasmid minprep kits, along with spin columns from an NEB gDNA extraction kit. The...
30 April 2025 7,997 1 View
Could any help me In the analysis of hplc-uv chrimatograms?( There Is a standards curves). Please contact me If Interested.
30 April 2025 6,262 0 View
With AI now creating art, music, and even inventions, traditional IP laws—designed for human creators—are facing major challenges. Who owns the rights to AI-generated works: the user, the...
29 April 2025 1,702 4 View
Hi, we are using the Epitect Fast Bisulfite kit from Qiagen for Bisulfite conversion. The Bisulfite has precipitated, which is posing problems. Tried heating the solution to 60°C with vortexing as...
26 April 2025 251 0 View
in my study high fat diet couldnot increase total chlesterol i have to discuss it and i require a references that indicate high fat diet can not increase total cholesterol?
26 April 2025 6,456 2 View
I have a pLEX 307 plasmid that has 3 BsmBI sites that I am trying to remove. 1 site is in the SV40 promoter, another site is in the PuroR sequence, and the last in the CmR sequence. I was able to...
25 April 2025 4,230 1 View
I am no longer receiving email notifications when researchers I follow publish new articles. How can I get them started again?
25 April 2025 2,392 2 View
I want to construct a phylogenetic tree using the maximum likelihood method and the phylogenetic tree will be constructed based on the gene. But I am not sure whether to use the DNA sequences or...
25 April 2025 9,443 2 View
Good afternoon dear colleagues. Anybody has the identification key for chigger mites "Kudryashova NI (1998) Chigger mites (Acariformes, Trombiculidae) of East Palaearctics. KMK Scientific Press,...
24 April 2025 5,786 1 View
What laptop specifications can run molecular docking soft wares?
23 April 2025 8,175 2 View
Hi there, I am really curious to know by what ways researchers from different background, especially those working in Computer Science or Computational biology, protect their research progress...
22 April 2025 2,283 4 View
How could YouTube provide IP rights protection?
20 April 2025 6,327 1 View
Hi, I am doing molecular docking using a 3D structure from PDB. This structure is from the rat protein but I would like to make sure the human protein would give the same results. Only two...
16 April 2025 4,754 1 View
In this attempt to resolve the conflict between the sexual nature of the human male and the pattern imposed by his code on the human female, the age-old institution of heterosexual prostitution...
16 April 2025 7,616 0 View
If the code works, and it replicates the geometry, it is a massive time saver. What are the prevailing doubts on this?
12 April 2025 6,602 4 View