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Questions related to Primer
I submited the lig_fix.mol2 file to genarate topology into a str file and got this messege error: "readmol2 warning: non-unique atoms were renamed. Now processing molecule LIG ... attype warning:...
18 July 2023 5,478 3 View
Hi! I'ts one of the first times i run SDS page for proteins and i got this result. what could be wrong? i attach a picture. thank you in advance!
17 July 2023 1,734 8 View
In a toxicology study, the EC concentrations are reported as as units of "log μM," for example 0.49 log μM. How do I translate that into simple μM concentrations? In this case, is the base of...
17 July 2023 5,556 2 View
I want know about pretreatment on sugarcane juice for fermantion and either yeasts generat eith microorganisms or externally added dried yeast.
16 July 2023 1,718 0 View
Hybridization is now common in plants and animals with the development of sequencing technology. Regarding the hybridization event, I have several questions that confused me. 1. I am confused...
15 July 2023 2,961 3 View
I am using a Qiagen Tissueruptor rotor stator homogenizer to homogenize brain tissue in TRIzol for RNA extraction. When I first began this protocol I did not clean some of the homogenizer tips...
14 July 2023 3,601 3 View
I wish to design a common LAMP primer for multiple sequences by primer explorer v 5. I tried to upload multiple files in software like clustal omega alignment, Notepad file with multiple sequence...
14 July 2023 4,663 2 View
Hi all, I am trying to produce an antibody from a plasmid in Freestyle CHOS cells. The plasmid is a HC/furin/p2A/LC vector. The cells were transiently transfected during the exponential phase and...
13 July 2023 2,390 5 View
Hi, i'm looking to expose my cells culture to ultrasounds. I need a way to expose the cells to 0.5MHz, 1Khz PRF with an intensity of 0.35-0.5MPa. Any suggestion?
13 July 2023 3,916 1 View
What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices? Can someone provide a study on the dose-response relationship of glutamate in brain slice preparations?
12 July 2023 9,545 1 View
I have prepared plant extract in methanol and ethanol and have preserved in DMSO solution
12 July 2023 3,759 1 View
Hi all, I have a technical question. There are plenty of commercial buffers which can be used as alternatives to commonly used blocking solutions (BSA, skim milk..) in Western B. or Elisa assays,...
12 July 2023 1,573 5 View
What are the innovative methodologies and experimental models currently being investigated in the field of pharmacology and toxicology research to comprehensively evaluate the long-term...
12 July 2023 6,349 8 View
Hello everybody, I purified my protein and when I run it on SDS-PAGE, a small molecular weight sized band (10 kDa) appears. Do you have any idea what that could be? an image of the SDS-PAGE is...
10 July 2023 5,305 3 View
I am a beginner with the use of SAS and Specially Orthogonal contrast. My experiment involve 4 rate of Nitrogen (23,46,69 and 92 kg N) at 3 time of application plus a control for bread wheat....
08 July 2023 2,368 13 View
Some sources indicate that after the onset of clinical symptoms (presumably, this would include prodromal symptoms), rabies postexposure prophylaxis (PEP) is of no benefit to the patient, and that...
08 July 2023 2,702 7 View
I want to measure the growth of a certain bacterium in rat feces. Therefore, I do not think it is necessary to use an NGS technique. Based on the literature for reading bacteria in feces,...
06 July 2023 1,105 0 View
How to approach a patient who was non-responsive to IM Penicillin and Oral antibiotics . Desppite being compliant with medicine and did not indulge in intercourse during and after therapy but VDRL...
06 July 2023 7,567 2 View
After immunoprecipitation with specific antibody and Protein G, samples were eluted with elution buffer, SDS sample buffer, and reducing agent at 70 °C for 5 minutes. Also, samples were incubated...
06 July 2023 8,936 3 View
I am looking at treatment as a factor with three levels; baseline, treated, untreated. My dds design included treatment, and genotype. Now i want to focus on the treatment contrast between treated...
06 July 2023 652 2 View
Hello everyone, Is there anyone working with lipid-based nanoparticles (LPNs) for mRNA delivery who can share their thoughts? Currently, I am working with PLGA:DOPE:DOTAP nanoparticles, but I am...
05 July 2023 7,550 4 View
I am a post graduate student in a NIgerian University. My project is on the genetic diversity of the pathogen responsible for decay in Cassava. Since the study is pretty new to me, i need guide on...
04 July 2023 4,943 5 View
I have run k-means clustering analysis in SPSS, but as far as I know I cannot test the stability of clusters in spss (my case count is over 11k). Which packages and code should I use to test...
04 July 2023 4,672 1 View
I want to determine the colocalization of a transmembrane protein along with a membrane marker, could it be possible to obtain the result with fluorescence microscopy alone or it is must to have a...
04 July 2023 2,670 5 View