OVCAR8 cells immunofluorescent stain with Hoescht and tdTomato - Contamination?

02 May 2025 1 9K Report

Has anyone seen something like this in Hoescht staining of OVCAR8? I am not sure if this is fungal contamination since it is in the cell and I see no sign of contamination in culture as the cells grow fine but have been finding this Hoescht network between cells a lot over the past couple of months of imaging?

Aaron Dhanda

sort of looks like mitochondria

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