For some reason I have a bit of a stockpile of spin columns from Qiagen plasmid miniprep kits, NEB Monarch plasmid minprep kits, along with spin columns from an NEB gDNA extraction kit. The buffers are essentially out. My lab is mainly undergraduate students so kits are my usual go-to for obtaining quality extractions. But, now we have loads of columns and no buffers.

I know I can buy replacement buffers for these kits but my supply budget is low.

So, I'm seeking out protocols for making my own buffers and using these columns. If anyone has some that work well (and avoid phenol/chloroform etc.) please share them.

My usual gDNA extractions are from frozen or dried leaves of domestic apple and hybrid poplar. I do have a 2-day column-free CTAB version that works well. I just want to use up the columns I have on hand.

Thanks a bunch! Looking forward to some assistance.