I have synthesized a cDNA. How can i check its quality, size etc ? can i simply run the normal gel or go for PCR it check these things ?

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Can Quran independently considered as a Source of Seerah(Biography of the Prophet Muhammad)?

How can one interpret and understand the Quran as a primary source for the life and teachings of the Prophet Muhammad, without relying solely on secondary sources or traditional commentaries, by...

05 July 2024 5,713 9 View

I have lyophilized primers.Do i need any buffer or PCR graded water to dilute my primers ?

primers are to be used for RTPCR

03 June 2024 8,588 8 View

Need suggestions for a topic related to General Surgery on which I can do a meta-analysis on using RCTs preferably?

The topic can cover any area of this specialty.

29 May 2024 3,200 1 View

Can phosphorylated and biotinylated oligos be stored at -80, or should be stored at -20? What is better recommendation to increase the life?

Oligo length is 19bp

10 January 2024 4,299 1 View

How to establish a Mathematical Model on "Transfer of heat in human body organs"?

I'm looking for a mathematical model for transfer of heat in human body organs especially (eye, heart, brain and lungs). How is the behavior of heat transfer at normal and extreme levels

12 July 2023 4,888 2 View

Discovery studio error while plotting the 2D structure of protein-DNA interaction ?

Hello everyone, I am using the discovery studio for the visualization of the Protein-DNA interaction as the 2D plot but it shows some error the ligand fragment is not selected, how I fix this...

21 June 2023 9,436 4 View

If anyone knows a numerical calculation for the emission of lighter when CO2 = 5.4‰ alters into δ13C = 3.1‰ in inflowing river waters?

If anyone knows a numerical calculation for the emission of lighter when CO2= 5.4‰ alters into δ13C = 3.1‰ in inflowing river waters? Thanks In advance!

04 December 2022 3,893 2 View

Is there any way how to to interpret LCMS data as we are getting only mass spectrum of the compounds in case of plant extracts?

I have done LCMS of different extracts of my plant.I got only mass spectrum of the compounds which is dfficult to interpret as there are different compounds whose molecular weight is same.They...

23 November 2022 7,968 5 View

Why sometimes CHARMMGUI does not generate lig.prm parametric file?

i am doing MD simulation for protein, 6lu7. when i use charmmgui to generate parametric files lig.prm file is not generated.

07 March 2022 6,739 0 View

Kindly suggest the solution for namd2 error FATAL ERROR: UNABLE TO FIND ANGLE PARAMETERS FOR CG2O1 CG2R61 CG2R66 (ATOMS 3946 3944 3950)?

i have been doing the molecular dynamic simulation for 6lu7 protein and some ligands and constantly getting the namd2 error.

03 March 2022 9,117 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View