How I can dissolve 3,3'diaminobenzidine(DAB) ?

02 July 2024 0 6K Report

I want to use a DAB dye to detect hydrogen peroxides in plant tissues, so I ordered the following product:

https://www.sigmaaldrich.com/DE/de/product/aldrich/d12384

The problem is that the DAB appears to be black crystallized and does not dissolve in any solvent I have used.

So far I have tried to dissolve it in 0.05M Tris-HCl, DMSO, EtHO, Distuled water and 3-5 drops of 37% HCl but no homogeneous dissolution.

What should I do, is it the product or am I using the wrong solvent?

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Science method

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