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Questions related to Plant Biotechnology
plant physiology, drought, wheat, soil saturation, biotechnology
07 July 2016 317 25 View
We have preformed numerous hydrolysis of soy concentrates (several different batches from different producers). We use standard conditions and mineral supplements. Last week one of the prepared...
07 July 2016 7,762 3 View
I need a good simple and easy protocol for measurement of ion leakage from plant root cells after heavy metal treatment. How can I measure the exact amount i.e. % of ions leakage ( need the...
07 July 2016 6,582 5 View
Preparation sample for SAM, samples were fixed with glutaraldehyde and then post-fixed with OsO4. OsO4 is extremely toxic to human and damage proteins and other components of sample. Could I skip...
07 July 2016 8,823 4 View
I am interested to check fluoride levels in hyper-accumulator plant tissues!
07 July 2016 832 3 View
Anybody knows from where I can download the sequence of this gateway destination plasmid pK7GW1WG2(1).O? Thanks in advance
07 July 2016 2,041 2 View
I'm working on micropropagation of Cornus mas cultivars. I have problem with regenerated shoots, The apical part died after 6 wk culture. I use a medium follow publication of Durcovic 2008. What...
07 July 2016 9,481 3 View
Dear friends, We are trying to isolate total RNA from pomegranate leaves, we tried using several kits but purity was the problem and it requires an additional manual purification for better...
07 July 2016 5,650 4 View
Importance of nucleosome in Chromatin remodeling
07 July 2016 5,110 2 View
Field survey, statistically representation with good correlation of present environment or weather etc.
07 July 2016 8,007 3 View
Dear all, As part of my study, I downloaded candidate genes for a particular gene family of Lotus japonicus and Medicago truncatula. Unfortunately, I encountered four pairs from Mt and six pairs...
07 July 2016 3,708 3 View
root and stem samples for short term storage, prior to sectioning
07 July 2016 7,720 4 View
Hi everyone , Can you please guide me what's the reason behind tomato plants are only generating the leaves and no shoots.
07 July 2016 2,634 3 View
I am completely new in field of Marchantia! Would like to grow marchantia on Gamborg media. What is widely used for this purpose, confused between sigma and duchefa?
07 July 2016 9,214 6 View
Need more information on chlorate seperation rather than removal.
07 July 2016 937 2 View
I want to quantify the jasmonic acid (JA) content in plant using HPTLC. I am following the method described by Dhandhukia and Thakkar, 2008. J. of Chromatographic Science, 46:320-324. After the...
23 June 2016 3,247 5 View
I am trying to extract total RNA using trizol from plant leaf. I just follow standart procedure as follow; Homogenizing the tissues (80 µg) within 1 ml trizol using Bead microtube homogenizer. Add...
06 June 2016 4,823 9 View
I know that cv. Tosca was a line variety and had not yields comparable with other cultivars from the same breeding period. But why was the work not continue with the "Tosca resistance"? Thank you
06 June 2016 3,206 2 View
can you tell me how i can estimate growth of suspension culture and tell me more about packed cell volume?
06 June 2016 2,470 3 View
Dear friends, Today I have got Atomic absorption data of my plant sample in which I have got one sample showing 497.1 microgram/L means in PPB and the Blank reading is 16 PPB Now I would like to...
06 June 2016 7,435 9 View
I am in plan to develop sweet corn from our elite germ plasm lines using the sweet corn genes Su1, Su2, Se1, Sh1, Sh2 and bt1, bt2. In this regard which genes combination will be better. Eg:1. Su1...
06 June 2016 1,625 9 View
Dear colleagues, I've got a recognizing problem with hexaploid wheat hybrids possible translocations in their chromosomes. After FISH, I've obtained in most cases 3 pairs of chromosomes (1B, 6B...
06 June 2016 1,841 3 View
Hi fellow friends...I'm interested in identify and isolating polyhydroxy alkaloid (imino sugars) from my sample (plant). I've gone through many journal and most of it use ion exchange resins in...
06 June 2016 4,204 3 View
I am going to start research on plant based protein materials. The works mainly involves extraction of protein from plant, its characterization, modification and applications. How is the...
06 June 2016 5,623 1 View