can you tell me how i can estimate growth of suspension culture and tell me more about packed cell volume?
Dear Pankj,
Please check these links for said query
http://www.nature.com/nprot/journal/v6/n6/fig_tab/nprot.2010.144_T1.html
http://www.biologydiscussion.com/plant-tissues/cell-suspension-culture/growth-patterns-in-cell-suspension-culture-and-its-measurement/14611
http://www.ncbi.nlm.nih.gov/pubmed/10567069
https://www.researchgate.net/publication/51187609_Initiation_growth_and_cryopreservation_of_plant_cell_suspension_cultures_Nat_Protoc
Good Luck
Article Initiation, growth and cryopreservation of plant cell suspen...
Dear Pankaj,
Please follow these researchgate links also.
https://www.researchgate.net/publication/258523455_A_simple_versatile_feeder_layer_system_for_Brassica_oleracea_protoplast_culture_Plant_Cell_Rep_9_316-319
https://www.researchgate.net/publication/228471469_Establishment_and_Optimisation_of_Growth_Parameters_for_Cell_Suspension_Cultures_of_Ficus_deltoidea
https://www.researchgate.net/publication/226196930_Establishment_of_embryogenic_cell_suspension_cultures_and_Agrobacterium-mediated_transformation_in_an_important_Cavendish_banana_cv_Robusta_AAA_Plant_Cell_Tiss_Organ_Cult
https://www.researchgate.net/publication/225500640_In_vitro_plant_regeneration_via_somatic_embryogenesis_through_cell_suspension_cultures_of_horsegram_Macrotyloma_uniflorum_Lam_Verdc
Best of Luck
Article A simple, versatile feeder layer system for Brassica olerace...
Article Establishment and Optimisation of Growth Parameters for Cell...
Article Establishment of embryogenic cell suspension cultures and Ag...
Article In vitro plant regeneration via somatic embryogenesis throug...
prepare 25ml medium and transfer your sample,use 250 ml Erlenmeyer flasks
after 2 week prepare25 ml from same medium again and add to same erlenmeyer
after two week divide each medium to 2 erlenmeyer and add to each 25ml fresh medium with same composition medium
use lower rotary for packing cell suspansion
https://www.researchgate.net/publication/289248357_Somatic_Embryogenesis_and_Plant_Regeneration_from_Juice_Vesicles_of_Mexican_Lime_Citrus_aurantifolia_L
good luck
Article Somatic Embryogenesis and Plant Regeneration from Juice Vesi...
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