Dear Pankj,

Please check these links for said query

http://www.nature.com/nprot/journal/v6/n6/fig_tab/nprot.2010.144_T1.html

http://www.biologydiscussion.com/plant-tissues/cell-suspension-culture/growth-patterns-in-cell-suspension-culture-and-its-measurement/14611

http://www.ncbi.nlm.nih.gov/pubmed/10567069

https://www.researchgate.net/publication/51187609_Initiation_growth_and_cryopreservation_of_plant_cell_suspension_cultures_Nat_Protoc

https://www.researchgate.net/publication/51187609_Initiation_growth_and_cryopreservation_of_plant_cell_suspension_cultures_Nat_Protoc

Good Luck

Article Initiation, growth and cryopreservation of plant cell suspen...

Dear Pankaj,

Please follow these researchgate links also.

https://www.researchgate.net/publication/258523455_A_simple_versatile_feeder_layer_system_for_Brassica_oleracea_protoplast_culture_Plant_Cell_Rep_9_316-319

https://www.researchgate.net/publication/228471469_Establishment_and_Optimisation_of_Growth_Parameters_for_Cell_Suspension_Cultures_of_Ficus_deltoidea

https://www.researchgate.net/publication/226196930_Establishment_of_embryogenic_cell_suspension_cultures_and_Agrobacterium-mediated_transformation_in_an_important_Cavendish_banana_cv_Robusta_AAA_Plant_Cell_Tiss_Organ_Cult

https://www.researchgate.net/publication/225500640_In_vitro_plant_regeneration_via_somatic_embryogenesis_through_cell_suspension_cultures_of_horsegram_Macrotyloma_uniflorum_Lam_Verdc

Best of Luck

Article A simple, versatile feeder layer system for Brassica olerace...

Article Establishment and Optimisation of Growth Parameters for Cell...

Article Establishment of embryogenic cell suspension cultures and Ag...

Article In vitro plant regeneration via somatic embryogenesis throug...

prepare 25ml medium and transfer your sample,use 250 ml Erlenmeyer flasks

after 2 week prepare25 ml from  same medium again and add to same erlenmeyer

after two week divide each medium to 2 erlenmeyer and add to each 25ml fresh medium with same composition medium

use lower rotary for packing cell suspansion

https://www.researchgate.net/publication/289248357_Somatic_Embryogenesis_and_Plant_Regeneration_from_Juice_Vesicles_of_Mexican_Lime_Citrus_aurantifolia_L

good luck

Article Somatic Embryogenesis and Plant Regeneration from Juice Vesi...