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Questions related to Plant Biotechnology
During plant tissue culture researcher using citric acid and ascorbic acid to minimise the browning problem of explant. Under which mechanism these two compounds prevents tissue from browning...
01 January 2017 6,598 7 View
I am trying to do tissue culture of Banana..So can anybody tell he how to follow the stages of positive initiation of culture from explants.. Like how the explants will look in the various stages...
01 January 2017 9,146 10 View
I will perform immuofluorescent labelling in Arabidopsis. For DNA stainig I can use SYBR Gold (there is no UV laser in the microscope). Does anyone have experience with SYBR Gold in fixed...
01 January 2017 851 0 View
I want to make a library for RNA-seq from a total RNA extracted from a complex system that includes eukaryotes (plant and fungi) and prokaryotes (bacteria). Does anyone have clues on how to...
01 January 2017 2,613 5 View
I wanna extract my expected protein which is targeted to nuclear in plant system. May I really need to add any detergent such as SDS or triton X100 in extraction buffer? Does PBS buffer is good to...
01 January 2017 373 4 View
I am looking for a vacuum pump to infiltrate solutions into plant tissues in order to fixate them (for molecular biology experiments like ChIP, in situs, etc…).I need something powerful, because...
01 January 2017 8,239 4 View
I am part of a research project that intends to carry out interspecific hybridization in Solanum quitoense, using wild species such as Solanum hirtum and the hybrid "La Selva". Thanks for your help
01 January 2017 5,979 2 View
If you take out your plant material from -80 and put on ice for 1 minute, and you realized that it is getting thaw and Put it back to the -80, Will it be good to extract RNA from that sample?
01 January 2017 6,093 7 View
I used Qiagen RNeasy Plant Mini Kit to isolate RNA from Turmeric Rhizome (6 months old) and used tertiary rhizomes as a sample for RNA isolation. I used two varieties (First Three columns...
01 January 2017 2,057 14 View
Hello Colleagues. I'm trying to study water movement in plants. Please, how can I inject a colored solution in vena plants?
01 January 2017 4,054 4 View
I have 2 genotypes, one resistant and another susceptible with two repeats of each genotype for stress and control condition. I will analyze them with one concentration of heavy metal at two time...
19 December 2016 8,605 7 View
Strawberry plants
13 December 2016 4,590 14 View
12 December 2016 9,702 13 View
An example is provided in this paper: doi: 10.1021/pr500196b
12 December 2016 2,388 1 View
Many papers use complicated methods to isolate microtubule associated proteins (MAPs), such as column chromatography and suspension cell culture, etc. Does anyone have a protocols that use easy...
12 December 2016 5,576 1 View
DE analysis was carried out using cufflinks, TAIR10 database, Arabidopsis
12 December 2016 2,089 6 View
method to determine protein content in plants.
12 December 2016 484 7 View
Hi friends, Can anyone suggest me good Promotercless plant expression vector? and if anybody used pGWB404 and pGWB405 vectors please tell me whetehr they are giving a good expression of...
12 December 2016 8,059 3 View
Recently I was performing some rooting trials with in-vitro produced walnut plants, at this time a lot of those plants have died from fungal infection. I mention some background about: - Induction...
12 December 2016 3,263 6 View
In cashew more litter was observed below the trees . i want to formulate on experiment regarding the decomposition experiment of cashew leaves (it contains more lignin, takes lot of time to decompose)
12 December 2016 9,661 4 View
12 December 2016 3,247 7 View
I want to innoculate plants with the 1 multiply by ten ki power 5 conidia per mL. How can I make this concentration?
12 December 2016 10,077 6 View
I am looking for an efficient protocol for extracting total RNA from olive oil or other plant-derived oils for human nutrition. Thank you for the support, kind regards.
12 December 2016 6,902 3 View
I am trying to genotype a SAIL line with the primers designed on the SIGNAL/SALK website. When genotyping known homozygous mutants, I am getting no bands with the LP+RP but a band larger than 1 kb...
12 December 2016 8,925 4 View