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Questions related to Plant Biotechnology
hi, actually, I am doing my research on determining heavy metal contents in plant roots. and the plant I used in my research is Balsam plant, which it has tiny hair roots. almost all the...
08 August 2016 3,390 15 View
Freezing stress can induce water loss due to the fact that formed ice breaks the membrane and electrolyte flux out of cell. However, chilling stress can also cause water loss.several reports...
08 August 2016 3,902 4 View
For a volatile bio-assay with Western flower thrips I'm looking for something that can extract or trap volatiles and then slowly releases them. Is Tenax suitable or is there a better option?
08 August 2016 8,237 2 View
I am working on an underutilized legume which is just about to be sequenced (African yam bean). It has a diploid ploidy level. Will the genome size have any effect on its genetic improvement?
08 August 2016 2,190 11 View
I used a wound inducible promoter (MPI) from maize. I transferred my construct to the tomato chloroplast. But I got confused, can the chloroplast RNA polymerase detect this eukaryotic promoter...
08 August 2016 8,149 20 View
Is anyone familiar with conducting iron uptake assay for filamentous fungi? I am trying to measure the concentration of iron in growth media in order to know how much iron (Fe3+ or Fe2+) is...
08 August 2016 8,040 3 View
By qualitative assay, we have found some bacteria showing positive to utilize nitrogen in Nfb media. Now we are trying to quantify nitrogen fixation by these endophytic bacteria. So if anyone...
08 August 2016 2,177 2 View
I extracted the total RNA from cyanobacteria and adding poly A using poly A polymerase, then synthesized cDNA using Thermo's kit. When using specific forward primer and oligo dT primer to amplify...
08 August 2016 2,340 1 View
Hello everyone I want to perform a prelimainary experiment for checking the resistance of six wheat varieties against wheat aphid under laboratory condition. For this I will sow 10 wheat seeds of...
08 August 2016 7,355 4 View
My friend said to me to use different levels of peclobutrazol and monitol on potato micropropagation. Is it ok, if so what is the process for that please.
08 August 2016 4,763 2 View
I am trying to deplete rubisco from total protein extract from rice leave,I have solubilised some samples in 8M urea and others are in pellet form. I have tried to do with SepproÒ Rubisco Spin...
08 August 2016 4,606 1 View
I need some opinions in terms of gene expression and secondary metabolites for plants grown under artificial light. I need to know if there are any big differences in terms of (anti-oxidant,...
08 August 2016 168 6 View
I am investigating the function of ACC synthase isolated from various vegetables such as pumpkin, melon, and cucumber. So, I initially isolated ACC synthase genes and purified ACC synthase...
08 August 2016 7,032 3 View
Hi, guys. I want to get a stablly expressed gene in cucurbit crops to get a suitable reference gene for qRT-PCR. Does anyone have any idea?
08 August 2016 7,187 0 View
Want to calculate the percentage of transformation in plant, using agrobacterium mediated genetic transformation.
08 August 2016 7,237 9 View
After going through some papers, I found that the liquid chromatography-mass spectrometric (LC-MS) is a good way for analysis of NA, but I have no access to LC-MS. Would you please suggest me...
08 August 2016 8,928 5 View
For transformations Its ok to use acetosryingon but for cotyledons after gemination putting on Pre-Cultural medium is it necessary. can you suggests me.
08 August 2016 1,585 2 View
Agricultural by-products mentioned in the question are sweet potato vines, sweet potato roots.
08 August 2016 2,726 3 View
I would like to go for mode of action studies for bio active compounds from plant source, for that it will be helpful if i get some studies involving methods other than, identification of membrane...
08 August 2016 6,021 5 View
I have been trying to clone Sugarcane genes in the pENTR/D-TOPO cloning system. However, for almost of my obtained Kanamicin resistant clones I don't have success in the insert confirmation using...
08 August 2016 2,182 3 View
In solid media for tissue culture of plants can any chemical substance be added that makes media porous and at the same time do not change media composition and pH? In solid media the plants are...
08 August 2016 2,482 4 View
Hi all, I need expert opinions on this matter as my plant samples are detached during washing step of FISH. I used agarose as well as transparent nail polish for this purpose and to me agarose...
08 August 2016 5,554 20 View
Need to the highest possible temperature that will give good quick results but not have a destructive effect on the constituents of the leaves. Kind Regards
08 August 2016 1,001 5 View
I am trying to clone a plant gene in PYES2 NTC vector, After Transformation i got colonies I isolated the plasmid from them and did double digestion to confirm, I got correct size insert release...
07 July 2016 4,863 3 View