I need a good simple and easy protocol for measurement of ion leakage from plant root cells after heavy metal treatment. How can I measure the exact amount i.e. % of ions leakage ( need the formula) against a standard.
To know the amount of solute a you can makes a standard curve with KCl, it probably what is recommended with the booklet that comes with your conductivimeter. Once you have measured the conductivity of all of your sample it's good to boil all your sample for 10 minutes that gives the total amount of solutes in your sample and may account for small sample errors like different sample size. The assay its self is easy, you just float your leaf or piece of leaf over ddwater for 1/2 hour.
Dear Dr. Germain,
Thanks a lot for your suggestions. I would definitely try. Can you provide a standard paper as reference to standardize the methodology?
Dear Dipan,
Please check the following articles:
1. Whitlow, T.H., Bassuk, N.L., Ranney, T.G. and Reichert, D.L., 1992. An improved method for using electrolyte leakage to assess membrane competence in plant tissues. Plant physiology, 98(1), pp.198-205.
2. Peever, T.L. and Higgins, V.J., 1989. Electrolyte leakage, lipoxygenase, and lipid peroxidation induced in tomato leaf tissue by specific and nonspecific elicitors from Cladosporium fulvum. Plant Physiology, 90(3), pp.867-875..
3. Murray, M.B., Cape, J.N. and Fowler, D., 1989. Quantification of frost damage in plant tissues by rates of electrolyte leakage. New phytologist, 113(3), pp.307-311.
Good Luck.
Measure the tolerance of the plant to the salt stress by the leakage of ions
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