I am completely new in field of Marchantia! Would like to grow marchantia on Gamborg media. What is widely used for this purpose, confused between sigma and duchefa?
I dont know what is Marchantia but for tissue culture experiments Duchefa chemicals are best choice.
Hi anamika, if you are dealig with vegetative propagation (i.e somatic embryogenesis ,organogenesis, callogenesis ) i would recomment you to go for Duchefa. with regards to pollen embryogenesis i will recommend sigma. good luck with your experiments
Hi Anamika,
here at SLCU we use the one from Duchefa and it works for both gemmae and thallus cuttings. I hope this helps!
Thanks for suggestions :)
By the way Giulia Arsuffi, shall I add vitamins and/or sucrose for routine cultures from gemmae? Is there any Marchantia web page with protocols and other basic details of the organism?
Hi Anamika,
most groups working with Marchantia do not add vitamins nor sucrose for routine culture, so I would start with that if it's the first time you work with it. I am running an experiment right now looking at whether vitamins and sucrose promote growth (alone or in combination), so I will get back to you on that soon!
Thanks a lot once again, and good luck with your expt. Looking forward to hear some exciting news from you :)
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