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Questions related to Plant Biotechnology
Recently I was performing some rooting trials with in-vitro produced walnut plants, at this time a lot of those plants have died from fungal infection. I mention some background about: - Induction...
05 December 2016 6,722 6 View
Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...
18 November 2016 9,410 7 View
When preparing MS media (distilled water, MS + sucrose, agar; adjusted to pH 5.8) I have noticed that, after autoclaving, there is a small amount of precipitate in the flask. It mixes back into...
15 November 2016 8,521 7 View
I need garlic seeds but usually garlic variety I grow do not flower
11 November 2016 6,814 6 View
Epidemiology of potato viruses
11 November 2016 1,718 7 View
I would like to measure photosynthesis rate using PAM-210 Chlorophyll Fluorometer but I don't know how to operate it. It would be a great help if someone share your experience.
11 November 2016 9,473 8 View
I want to do the viability assay using MTT salt in a plant cell, but I don't know how I can count these cells, can I use the same method to the human cell?
11 November 2016 8,142 4 View
Plant Development, Plant Biology, Tissue culture, Hormonal signaling,
11 November 2016 2,705 6 View
there are many constitutive promoter but expression level in all tissue is not equal. any promoter which also give high expression level in seed and pollen? if any relevant paper then please...
11 November 2016 4,636 3 View
multiplication stage, shoot regeneration, calcium nitrate, media
11 November 2016 9,004 3 View
11 November 2016 4,562 8 View
I have been monitoring the δ15N of two species of floating plants over 5 concentrations of NO3 and NH3. The measurements have been done over 16 days, with solution changes every 7 days to avoid...
11 November 2016 7,332 4 View
agriculture, biotechnology
11 November 2016 5,839 3 View
I have a question about WiscDsLox mutant and WiscDsLoxHs mutant, it seems they are different? i have a WiscDsLoxHs mutant, and i used p745 T-DNA primer to check if the plant is homozygous or not....
11 November 2016 9,343 7 View
cotton
11 November 2016 2,159 10 View
I have to test different primers concentration for Arabidopsis thaliana genes and its mutant by RT-qPCR. I made 4 different stock primers concentration: 800nM, 1000nM 1200nM and 1400nM. In final...
11 November 2016 8,830 6 View
Like One gram of shoot and root tissue need to homogenize in 4 ml of 100 mM potassium phosphate buffer (pH 7.4) containing 1 mM ethylenediaminetetraacetate (EDTA), 2 % polyvinylpyrrolidone, and 1...
11 November 2016 5,974 3 View
I am currently trying to use green peach aphid(GPA), potato and potato viruses to conduct an experiment.I have a Potato Leafroll Virus infected potato (Superior) and GPA colony grown from tobacco...
11 November 2016 6,685 3 View
I am working with Combiness Microboxes filled with granulated clay that is watered with half-strength MS-medium (w.o. sucrose). We use them in our lab to raise axenic or gnotobiotic plants....
11 November 2016 9,415 4 View
11 November 2016 1,386 5 View
I am currently working on Paddy type MFC, which has been operated already for more than 3 months, It is nearly a time to harvest, I am getting negative voltage in rice planted MFC since few...
18 October 2016 3,810 3 View
I followed several papers using ammonium molypdate method but there was precipitation and the results were inconsistent. The protocol is as followed: - Extract freeze-dried leaves with HClO4 10%,...
10 October 2016 1,768 12 View
Hi. I would like to check the differential expression of two versions of this gene, one of this containing an insertion in the upstream. I planned to transform Arabidopsis with the whole gene (not...
10 October 2016 6,047 8 View
Anyone working on anther culture or any tissue culture related work on pomegranate... I have a major issue regarding phenolic production in culture which becomes almost black even after using...
10 October 2016 9,765 5 View