Discussion of the plant sciences | Contact experts in Plant Biology to get answers
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Questions related to Plant Biology
For a while I am searching on internet for types, material and especially: mesh-sizes of outdoor-proof insect netting for emergence/breeding-experiments. Its to breed a complete community from...
21 March 2024 7,229 1 View
I have an experiment testing efficiency of different concentrations of a herbicide mixed with different concentrations of surfactants (ex., 0.5 g/l herbicide + 0.0125 g/l surfactant, 0.5 g/l...
07 September 2023 1,826 1 View
Open pulled straws which are made by manually pulling heated French mini straws usually get contaminated during processing and after that how they can be sterilized. I mean the method recommended...
10 November 2020 3,903 3 View
Dear all, Kindly explain these two primers, Are these forward or reverse or different types of primers? Here is the statment from the paper [The Internal Transcribed Spacer region (ITS) was...
20 July 2020 8,182 0 View
I had tried surface sterilize the moss but it came out with fungal contamination...high concentration of surfactant turned out yellowed moss and dead...does anyone tried before?
17 February 2019 1,780 1 View
By performing a peel test with the Universal Testing Machine (UTM) the peel strength or adhesion strength between two materials can be easily determined. But I would like to know if there is a...
23 October 2018 1,146 0 View
This is my understanding. If it is wrong, please correct me. So animals have an adaptive immune system where when they come into contact with a foreign substance they create antibodies to it and...
20 October 2018 673 11 View
I am trying to distinguish glucose levels in the apoplasm as well as in the cytosol of Arabidopsis leaves. Clear protocols exist for apoplastic glucose quantification. However, I have some hard...
23 August 2018 8,917 0 View
Hello, I tried hard to keep everything sterile, but I can still see some black particles after collagenase treatment for 2hours.... is there steps I could be doing wrong that allows bacteria to...
13 August 2018 5,636 15 View
In this video the girl puts a cos lettuce in boiling hot water. The lettuce does not wilt. She then starts to peel off strips of plastic like material from the back of the lettuce leaves. Could...
27 July 2018 877 4 View
How many chlorophyll molecules are involved in photosynthesis?
03 June 2018 4,407 8 View
I am using temperature profile Oven temperature initial 50 degrees for 30s then raised to 80 degrees at the rate of 10degrees/min hold for 30s then raised to 150 degrees at the rate of 10...
11 May 2018 5,001 3 View
I'm searching for 2 scientists to share in writing a research proposal entitled "microbial source of water for the desert plants" to be submitted to "Water JPI 2018 Joint Call", from any of the 20...
16 March 2018 8,182 5 View
The conductivity of the chloroplast ATP synthase is usually estimated from the time constant of a first-order exponential fit to the post-illumination relaxation kinetics of the electrochromic...
01 November 2017 5,678 1 View
I am experimenting on the effect of salinity in Arabidopsis. I had worked with the same brand of MS medium before, and the seedlings had grown green as usual. But then I used another package of...
02 August 2017 8,148 5 View
Hi there. I'm doing some work in Sodankyla, Finland which is quite high north and a bit remote. I need to get frozen/refridgerated samples back to the Netherlands. I'm limited in my materials to...
28 July 2017 4,693 3 View
Our research is plant biology and Arabidopsis is the main plant material. But during a long time, our plants endured serious damage by aphid. Do you have some good suggestion for me to reduce the...
30 June 2017 1,075 31 View
Hi All, I have some compounds and I'd like to examine how they effect the expression of nitrogen transporters in maize (corn) roots. My plan is to grow corn in a defined media (i.e. Yoshida's) in...
29 June 2017 4,761 2 View
I am growing a BSL1 E. coli strain (ATCC 8739) for use in biofilm studies with a drip flow reactor. I am a chemist/materials scientist by training, so microbiology is pretty new to me. I have...
14 June 2017 8,078 4 View
I want to do Root Organ Culture of carrot. I have tried with agrobacterium rhizogenes mtcc 532. i have tried with 8hrs, 12 hrs culture, 24 hrs, 48 hours culture.. i have collected cells by...
13 March 2017 4,899 5 View
I have a data set of chemical and physical traits (and herbivory data) from trees and vines at one of my study sites. Unfortunately, there was no overlap in the families trees vs vines came from...
27 February 2017 3,548 3 View
I am working on Fenugreek (legume).
25 January 2017 579 4 View
How can i determine Callose in Citrus Plant roots?
11 January 2017 768 8 View
From many related scientific papers, it is stated that the Water (harsh non-solvent) is added slowly to the transparent PVDF/DMF homogeneous solution (which is constantly stirred and kept at fixed...
06 January 2017 2,457 2 View