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Questions related to Plant Biotechnology
we placed plant in a dark conditions (in growth chamber without light) before isolation of mtDNA but still we have little contamination of chloroplast DNA. Is there any other method to control it..
10 October 2016 3,996 6 View
Please can any one suggest me preparation or how to dissolve gingkotoxin for my experiments?
10 October 2016 4,910 4 View
Plant Breeding, Genetics, Statistics
10 October 2016 2,704 3 View
I have tried transforming A. thaliana and N. tabacum plants using Agrobacterium mediated co-cultivation/culture method but in case of A. thaliana, I could not get any callus after agroinfection...
10 October 2016 4,705 19 View
Hi, I'm purifying a recombinant protein. It's secreted protein. After chromatography procedure, I have some samples of protein: - Fermentation sample (applied sample): the supernatant from BSM...
10 October 2016 1,180 12 View
I am looking for any research, published or not, that can explain quantities of mineral nutrients that may increase terpenoid synthesis. Are there any organic or naturally-derived supplements that...
10 October 2016 6,801 4 View
I have to isolate more than 2500 wheat leaf samples for screening. Even we use robotic system, dna isolation is very time consuming and costly. We will use only 2 primer pair for screening. I wish...
10 October 2016 4,782 4 View
I did AFLP with fluorescent primers by Applied Biosystems ( EcoRI-ACT-FAM(402045); EcoRI-AGG-JOE (402043); EcoRI-AGC-NED (4303057)). I would like to continue my work, but primers have been...
10 October 2016 464 4 View
I am currently working on Paddy type MFC, which has been operated already for more than 3 months, It is nearly a time to harvest, I am getting negative voltage in rice planted MFC since few...
10 October 2016 4,290 3 View
Hello all, I'm looking an adjustable multichannel pipette for our studies. There are some alternatives mettler toledo and voyager brands. Is there any experienced with these brands? Or any...
10 October 2016 7,029 2 View
I am doing RNA extraction using Trizol Reagent in vitis. but 260/280 1,00-1,35 and 260/230 0,1-0,3. ı have only chemicals for trizol method. Do ı have to change to method or may ı make to modified...
10 October 2016 8,438 4 View
Read 8 answers by scientists with 11 recommendations from their colleagues to the question asked by Dr. Tresina Soris P on Oct 11, 2016
10 October 2016 3,826 8 View
We are looking for a portable photosynthesis system which is able to measure photosynthesis in Arabidopsis seedlings with very small leaves.
10 October 2016 348 6 View
Hello, I would like to remove selection cassette from my mutant plant in order to transform another construct with same selection. I know, cre/lox recombination is one of the possibility, but...
10 October 2016 2,603 7 View
I am working on nitrogen-fixing fresh and marine water cyanobacteria. I want to estimate proline. Whether I should standardize the OD to ensure the exponential phase or the time limit for growth...
10 October 2016 5,850 3 View
when i use the method of 4 amino there is formation of micelles
10 October 2016 3,050 2 View
1. why we grow citrus rootstocks seed in dark. Is there any advantage to grow it in dark. 2. what possible difficulty I have to face if I grow the grafted plant in solid medium instead of liquid...
09 September 2016 8,019 9 View
Laminar flow hood used for Plant micro propagation
09 September 2016 8,425 5 View
Hello, can someone suggest me which antibiotics are best to get rid of Rhizobium bacteria? And also at which concentration? I would like to add the antibiotics on agar plates and remove bacteria...
09 September 2016 5,601 4 View
Hi all I wish to optimise my antiinflammatory fraction of plant to successful lead against inflammation, After separation and optimisation insilico how to progress with and invivo evaluation of...
09 September 2016 579 3 View
Citrus medica was chosen to explore the potential of citrus fruits in reducing agents.
09 September 2016 2,980 3 View
Hi all I am working on cross pollinated crop of Jatropha Curcas. in this i want to map the QTLs for yield related traits, to this I have selected two inbred lines at self generation 6(S6) level...
09 September 2016 4,854 7 View
arabidopsis thaliana
09 September 2016 4,389 3 View
I want to see plant RNA virus under SEM
09 September 2016 4,979 3 View