Contact experts in Plant Biotechnology to get answers
3,001 views 1,162 posts
Questions related to Plant Biotechnology
As the rhizobium responsive gene may be an impediment for legume's responsiveness to inorganic fertilizers
07 July 2016 3,695 18 View
.
07 July 2016 556 1 View
I'm looking for a simple method for measuring interstellar- cytoplasmic pH of plant cells.
07 July 2016 6,620 8 View
Plant Tissue Culture (PTC).
07 July 2016 4,545 10 View
I want to design primers to amplify a gene in genome specific manner in wheat which is present in all A, B and D genomes with high similarity. Because of the high similarity, facing problem in...
07 July 2016 4,092 7 View
I want to assay caffeic acid in ocimum basilicum (sweet basil). What is the best methods for assaying caffeic acid in plant tissues?
07 July 2016 4,124 3 View
The transition from cell division to cell elongation is usually 30 to 50 days in citrus, but how about the transition from ovary to fruitlet in citrus? How much time dose it need to finish this...
07 July 2016 6,160 2 View
Can someone share agro bacterium mediated transformation protocol for pCAMBIA 1305.1 in wheat please? I am using Bobwhite cultivar and C58C1 agro strain but the regeneration rate is very poor.
07 July 2016 8,619 2 View
Dear Researchers, Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought...
07 July 2016 5,052 3 View
I'm conducting an experiment to evaluate the effect of different humidity levels on a specific treatment of grains (rice grains), I'm looking for a reliable method to control the relative humidity...
07 July 2016 4,188 7 View
Hi, I'm studying in the fermentation of P. pastoris. I try to find out what difference between BSM and BMGY. I just know the elements of those media, but I don't know the role of each element....
07 July 2016 5,883 2 View
I am extracting RNA from barley leaves and my gel photos are all like these ones. The problem is that other people used the same method for this plant and they look to not have a problem with...
07 July 2016 626 14 View
Please suggest me what should be done in case I'm doing random integration of my protein localization construct in a fungus by means of ATMT (Agrobacterium Mediated Transformation) where I don't...
07 July 2016 5,859 4 View
I have cloned my target gene (From plant) in pET32a but i cant transform it in BL21 (DE3) cells. I tried many times its not getting transformed. Even i tried along with pLYS S but i am not...
07 July 2016 9,151 2 View
Hello. I have a conceptual question for the community today. If root lengths of two different genotypes in Arabidopsis are significantly different and they are showing a difference in LR emergence...
07 July 2016 1,631 3 View
if Xylella fastidiosa transmitted by Edwardsiana rosae, between what host.
07 July 2016 2,198 4 View
Mitochondrial membrane potential of plant cells is easily detectable using fluorescent microscope. If I want to quantify the changes in fluorescent intensity, how can I detect it?
07 July 2016 1,822 1 View
I need to bring together some factors of development of mycorrhizae arbuscular after inoculation in plant host or before
07 July 2016 950 4 View
particularly the range of irradiation dose exposure
07 July 2016 6,080 2 View
For GFP localization study by confocal microscopy, I need to prepare a permanent slide of the protoplast samples which are prepared from the agroinfiltrated leaves of Nicotiana benthamiana. Is...
07 July 2016 6,055 7 View
how
07 July 2016 6,401 14 View
Hi, i have isolated RNA from banana and get a high purity and concentration, then converted it to cDNA and amplified with spesific primer but not appear on electrophoresis gel. how to solve that...
07 July 2016 1,461 6 View
I am doing an experiment using tomato seedlings. The plants used as starting material for the experiment are chosen as uniformly as possible from a larger population of seedlings. As the standard...
07 July 2016 7,500 3 View
I am working on Boraginaceae family. Now I have n-hexane fraction, I want to run column for this fraction. From which solvent system should i Start?? And then at what rate I change the polarity?
07 July 2016 9,009 2 View