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Questions related to Plant Biotechnology
I am working with the aphid and nematode resistance genes in the soybean. I want to utilize the already generated SNP markers of the soybean genome in SoySNP50K iSelect BeadChip from soybean...
09 September 2016 7,580 3 View
Plant Biotechnology, Plant Genetics,
09 September 2016 3,175 8 View
These samples are meat balls with some structures. The idea is someone that work with taxonomy of caterpillars. I have photographies of these samples and if someone have interest, I could send...
09 September 2016 7,446 3 View
Dear Researchers, Im trying vacuum infiltration for seed transformation of a recalcitrant plant. I tried different time durations of vacuum infiltration (1 min to 10 min), culture density...
09 September 2016 6,802 3 View
I want to compare two set of data containing 8 observations and even though they follow a normal distribution, I am not sure it's correct to perform a student-t test on it because the set of data...
09 September 2016 3,271 11 View
I did touchdown PCR using gene specific primer targeting lectin gene inserted into the cotton genome by isolating DNA from T2 plants.The PCR showed positive result in one of the event plant. The...
09 September 2016 5,503 5 View
I would be interested in blocking mRNA degradation in Arabidopsis. Someone would know some drug? Thank you!
09 September 2016 5,023 4 View
Hello everyone, I cloned three Arabidopsis thaliana Argonautes proteins (AGO5, AGO10 and AGO2) into pEAQ vectors. They are tagged with mCherry in N-terminal. When I agroinfiltrated benthamiana...
09 September 2016 2,462 9 View
I am attempting to knock-out the gene F3'H in Arabidopsis (NC_003076.8). What is the recommended length of the gRNA? I understand that it should be 17-20nt long (+PAM), but which length will...
09 September 2016 5,662 5 View
Dear, I make competent Agrobacterium with cacl2 and do transformation with them. Finally I take colonies in plates but they are rough and dry and separate on lb agar and not mucilage? Why it happen?
09 September 2016 3,117 7 View
I want to study conformational / structural change in the protein after ATP binding ( I am working with a yeast protein that shows ATPase activity) so I want to see and compare the binding effect...
09 September 2016 4,454 4 View
can you also suggest the procedure of mutation in in-vitro conditions and level of mutagens?
09 September 2016 2,353 4 View
I have done hematoxylin staining of plant nucleolus (eg. alluim) now would like to do silver nitrate staining. So I need a simple and standard protocol to see the effects of heavy metal treatments...
09 September 2016 616 0 View
what is the latest research on Ulmus micropropagation?
09 September 2016 3,558 2 View
Hi, How can insert my all sequence of my WRKY TFs in vector- E.coli: I want start to study the function of my WRKY gene to abiotic stress (nitrogen) but I have problem because I want insert all...
09 September 2016 2,322 2 View
Hi everyone, Currently, I am doing ChIP assay using EpiQuik Plant ChIP kit from Epigentek. At step "Antibody Binding to the Assay plate", the kit instruction says that the strip wells must be...
09 September 2016 501 3 View
How can be CRISPR Cas 9 technology applied to manage insect teansmitted plant pathogens with special reference to insect transmitted plant virus? Literatures or previous work done on the above...
09 September 2016 2,505 3 View
It is confuse to handle monoculture of microalgae in laboratory.
09 September 2016 8,206 4 View
Hi, How to find full length sequences of MYB genes of wheat?
09 September 2016 8,783 7 View
We have isolated a Xanthomonas from Salvia with leaf spots. The isolate could not be identified yet, as it was not amplifyable using the gyr primers of Parkinson. It was identified as X. sp. via...
09 September 2016 7,562 10 View
I have used Full strength of MS and Half strength of MS media along with BAP and 2,4 -D , with and without Charcol(.5mg/L/) and for sterilization I used Ethyl alcohol and HgCL2 . But no...
09 September 2016 6,823 5 View
I have Vector containing Ubi promoter and I want to use it in bacteria .
09 September 2016 10,035 1 View
There are several culture techniques for culturing microalgae, such as batch, continuous, semi continuous culture. If I want to get high biomass (until early stationary phase) and then I want to...
09 September 2016 2,154 6 View
if is there any references would be very helpful.
09 September 2016 1,253 1 View