Plant Biotechnology

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Questions related to Plant Biotechnology

Recommended books on plant breeding and genetics?

Hi, I'm new here so sorry If I'm in the wrong place. I've been working in UK Agriculture for around 10 years now and I've decided to pursue a career in R&D and plant breeding after working...

31 March 2018 2,439 21 View

Recommended books on plant breeding and genetics?

Hi, I'm new here so sorry If I'm in the wrong place. I've been working in UK Agriculture for around 10 years now and I've decided to pursue a career in R&D and plant breeding after working...

03 March 2018 9,459 18 View

CRISPR/Cas9 vs CRISPR/Cpf1?

Which is much better (especially for plant genome editing purpose), CRISPR/Cas9 or CRISPR/Cpf1?

13 February 2018 2,480 5 View

Can we feed the world in 2050?

As we all know world population will rise from 7.5 billion to 9.5 billion in 2050 and that will increase the total global food demand from 50 % to 97% according to FAO . What is your perspective...

02 February 2018 8,528 3 View

Is there any parameters in a plant that we can measure to identify the current growth stage of the plant?

Hi. We are currently working on a project that aims to supply the light needed by the plant. We come across a research that the growth phase of the plant i.e. seedling stage, vegetative stage,...

08 January 2018 1,169 8 View

Does this abstract report any findings?

I would like to request researchgate people to comment on the following abstract. Does this report any findings, or convey a message? What are the weakness of this abstract? Improved design of...

10 December 2017 7,277 4 View

Postdoctoral research opportunity?

Dear All Looking for postdoctoral research opportunity in plant biotechnology/molecular genetics. Any suggestions/guidance? Thanks

11 November 2017 9,967 0 View

What is precision plant breeding?

Precision farming has become popular to everyone associated with advancement in farming strategies. Similarly, in plant improvement what is the scope of precision breeding? I think, it possess...

11 November 2017 6,939 13 View

L4440 Plasmid, Is there any researcher who can provide L4440 plasmid to me as I require it for my PhD work?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

10 October 2017 9,937 0 View

I want to use empty vector as a control to my transgenic plants . how can it be proceeded?

plant biotechnology and molecular biology

08 August 2017 9,104 1 View

Why we use half strength MS media for root induction from micro shoots

Invitro raised shoots were transferred to half strength MS media for root induction and what is the role of ammonium nitrate strength?

07 July 2017 5,947 7 View

What is the relationship between lanthanoids and plant biotechnology?

Is there any relation corresponding to the plant growth regulators? Is it issue of toxicity?

04 April 2017 6,818 18 View

Yeast vs Plant cell - which is better for the production of recombinant proteins?

Kindly elaborate on pros and cons of both, if possible. I'm interested in understanding the differences in levels of recombinant protein produced, ease of production and ease of genetic...

03 March 2017 828 10 View

Can i use Oryzalin to produce polyploid Begonias ?

Begonias are sensitive to Oryzalin herbicide and it is not used for Begonia Polyploidy studies and i doubt if Begonia mini cutting can survive Oryzalin Treatments . I want to use this article as...

11 January 2017 7,715 4 View

Does chloroform melt ependorff or other larger centrifugr tubes?

Hi all! While doing my DNA isolation I encountered a new problem. After the addition of chloroform and during centrifugation, the ependorff and 50mL centrifuge tubes melted. Can anyone provide me...

01 January 2017 1,540 13 View

Problem in DNA extraction?

Hi, How i can remove polysaccharides and polyphenols during the DNA extraction from Terminalia arjuna, i have tried various methods but unable to find DNA in purified form? I have tried many...

01 January 2017 6,034 3 View

Pollen germination media of pumpkin?

what is the best combination of media and procedure that could help in identifying the viable/germinated pollen of pumpkin. We experienced bursting of pollen that's why we cannot identify wither...

01 January 2017 3,400 3 View

Any tool to predict the sub-cellular localization of a list of genes without needing full sequence information?

I have lists of genes obtained from a differential expression analysis in tomato. I would like to use a tool to predict the % of differentially expressed genes having a predicted signal peptide to...

01 January 2017 9,300 2 View

Looking for a single gene mutant reference?

I am looking for a reference in which a single gene mutant having weak phenotype when combined with another single gene mutant having strong phenotype of the relative trait, result in a weaker...

01 January 2017 8,131 7 View

Which is the best Reference/ control gene for expression profiling in case of Plants?

Hello everybody , Can anybody guide me about the best gene for plants as control for expression profiling(qPCR). i m working on Coleus forskohlii ABC transporter proteins

01 January 2017 9,775 7 View

Can i use Oryzalin to produce polyploid Begonias ?

Begonias are sensitive to Oryzalin herbicide and it is not used for Begonia Polyploidy studies and i doubt if Begonia mini cutting can survive Oryzalin Treatments . I want to use this article as...

01 January 2017 3,123 5 View

What should be the ideal extraction buffer composition for plant total protein isolation

I am using the extraction buffer for plant total protein isolation for MALDI. the composition of te buffer is Na- Phosphate buffer (PH 6), 1mM EDTA, glcerol, tritron x, PVPP, 2-Mercaptoethanol,...

01 January 2017 1,895 6 View

Has anyone got an idea about separation of inner and outer chloroplast envelope membranes from Nicotiana benthamiana?

We could isolate intact chloroplasts, but we are facing difficulties in obtaining outer and inner envelope membranes on a sucrose gradient.Suggestions are welcome

01 January 2017 9,831 3 View

Which Agar is better to keep fresh a leaf disc in peti dish for 48 h?

I want to prepare leaf discs of cucumber in agar medium in petri dish so that just leaves stay fresh to 48 h. Which Agar is better?1-Agar agar 2-plant agar 3-bacteriologic agar

01 January 2017 5,129 10 View

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