Iron Uptake Assay for Filamentous Fungi?

Is anyone familiar with conducting iron uptake assay for filamentous fungi? I am trying to measure the concentration of iron in growth media in order to know how much iron (Fe3+ or Fe2+) is...

07 August 2016 7,950 3 View

TB3 is the abbreviation of what?

TB3 is a common media used for the regeneration of protoplast.. Anybody knows what is it abbreviated for? If you have a reference for it, kindly share with me... Thank you

04 May 2016 1,273 1 View

Expression of a fungal gene under the control of it's own promoter?

I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...

04 May 2016 687 7 View

Online promoter and terminator prediction tool?

Is there any online tool available for predicting promoter and terminator sequences within the mRNA sequence?

02 March 2016 4,999 5 View

Why do i have smears on gel after restriction digestion with 2 enzymes?

After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...

02 March 2016 8,451 9 View

If AGL1 Agrobacterium is transformed with pGKO2 vector without gene insert, can it grow on LB medium with kan antibiotics?

Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...

08 September 2015 7,130 5 View

How can I control the growth of unwanted spores in the Verticillium dahliae culture?

We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...

02 March 2015 3,549 17 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View