Hello! I'm new to using AFM to analyze nanoparticles in a solution, and I have a question. I see a "line fit 2.79 nm" bar on the right side of my image. Does this refer to the height of the features in the image? For example, does the white area correspond to an approximate height of 2.79 nm? I noticed in another sample, which doesn't have nanoparticles, that it shows "line fit 700 pm." From this, I’m guessing that this value might represent the maximum height. Could you confirm this? Thank you!
1) Before we discuss heights, let's talk about the flattening: the lines you see around the larger particles indicate that there are flattening artifacts which would give you a false profile. These can be excluded in Nanoscope directly during the flattening dialogue while in Gwyddion you would have to perform a zone selection beforehand.
2) Regarding what you see: you would have to check what the recording channel is called because an output like this can come both out of a height sensor and an amplitude error channel. If you work with Gwyddion, you can see the channel name under Info/Data Browser.
Andreea Iosageanu Jürgen Weippert Hi, can you please look at this question and share your thoughts?
https://www.researchgate.net/post/How_to_target_micro_particles_using_AFM_Contact_mode2
Jürgen Weippert Thank you for your reply! I have access to Gwyddion, I will explore that further!
Hassan Liaquat I am by no means an expert and am still in the process of learning about AFM. Why aren't you placing the nanoparticles on freshly cleaved mica? Wouldn't that help keep them in position? This is the method I used to prepare my samples.
Andreea Iosageanu The particles i am using are microbeads .. which needs some liquid to maintain its shape , but they keep swimming in that liquid droplet.
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