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Is glycerol enough as a mounting medium without mixing with PBS for immunofluorescent techniques for frozen sections, and what is the difference from using glycerine in replacement?
14 October 2019 2,846 9 View
I like to know the possible markers of metabolism in cancer cells such as in monitoring glycolytic, extracellular acidification and oxygen consumption rates, effected by treatment with an extract.
25 September 2019 808 3 View
How do you advise a group of authors whose papers already accepted but only listed for several months under ahead-of-print without publication as necessary?
23 May 2019 3,611 5 View
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16 February 2018 9,773 3 View
Which one under- or overestimates significance in the test of association?. Often times, I come across both choices in the selection of SD and SEM as a measure of spread, however, little is...
09 February 2018 5,081 1 View
Proposed substrate is sawdust from sawmill. Target mushroom is oyster mushroom
22 February 2017 6,265 5 View
I am trying to study the hydration of hydrated cementitious binder at different days of curing. The samples are in 40 x 40 x 160 prisms. I intend slicing them into 8mm thick samples. If these...
23 October 2016 7,908 14 View
If formaldehyde has been exposed for 1/2 days for the purpose of fumigating a mushroom farm, how should it properly be disposed? Any negative impact if it is disposed inside farm house wash hand...
13 February 2016 9,538 8 View
For instance a Volvariella volvacea fruiting body growing on spent P. tuber -regium substrate without inoculating the spent substrate of P. tuber regium with V. volvacea.
30 November 2015 3,384 4 View
I have been working on earth pressure magnitude and distribution in jointed rock mass and I need to use slope mass rating to analyse the results.
28 January 2013 6,852 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
