Hello,

I am trying to culture a specific subset of macrophages from murine pancreas, but they do not grow well in cell culture with RPMI-1640 (+FBS + M-CSF). I want to try growing them with some sort of media that is made from the pancreas itself. However, I cannot find any protocols how to make a pancreas extract media. Any advice?

I was thinking of mechanically and chemically digesting a mouse pancreas, spinning and filtering to remove cells and debris, and then mixing 1:1 with RPMI-1640. I was worried the pancreatic enzymes and the chemical enzyme leftover from the digestion might be toxic to the cells, though.

Thank you,

Seth