How many reads should I use when sequencing 18S rRNA, 16S rRNA and ITS2, using Amplicon Sequencing ?

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How long to treat male mouse to affect the offspring ? When is the paternal programmation efficient ?

My question is mostly about how many spermatogenesis cycle does it take so all the spermatozoa are effected by a deficiency / treatment, and so the offspring is completely affected ? No matter if...

10 January 2024 7,099 0 View

What is the 70kDa band in Western blot of full mouse heart?

Dear I performed Western blot on full mouse heart lysates of MHC-cre mice. I tested 2 different genes, and in both Western blots I had an unexpected band at 70kDa. Does someone has an explanation...

16 November 2023 7,864 0 View

How long does it takes to digest ~20mg of tissue with Proteinase K at 56°c ?

I'm preparing DNA extractions after digesting my 345 samples with PK, but I'm wondering how long i'll take to digest each tissue If anyone has a precise idea about how long will it takes, within a...

02 August 2023 6,565 1 View

Is it possible to display, percentage of limitation speed reached on PVT Vissim ?

Usually when a speed map is displayed on PVT VISSIM 21, a color is associated to each link depending on the average/maximum/minimum speed on this link. Is there a way to display a color depending...

03 April 2023 8,921 0 View

Is it possible to stain cells after flowcytometry?

Hi all! I'm purifying endothelial cells with FACS, but to confirm the cell population after FACS, I want to stain the sorted cells to visualize them under the microscope. So far, no succes. Does...

08 February 2023 7,389 2 View

Strange immunostaining of P-gp on Caco-2/TC7 cells ?

Hello Scientific Community, I need your help, I did an immunostaining of P-gp on Caco-2/TC7 cell line. I used a classic protocol with 4% PFA fixation and without permeabilization. You can...

01 February 2023 4,000 3 View

What are the best standardized tests to establish cognitive abilities/profile of participants with complete blindness or severe visual impairment?

I work with people with complete blindness, and tasks are cognitively demanding. While I made sure our participants did not present comorbidities and cognitive impairments, I am looking for...

23 January 2023 6,396 4 View

How to perform Western blot on low cell count?

Hello I am working on a protocol to perform Western Blot on sorted endothelial cells from the liver. So far, I tried to do protein isolation in NP-40 buffer with 120.000 cells but I do not get...

07 November 2022 8,543 4 View

Evan's blue dye is a relevant technic to verify the blood brain barrier integrity after viral infection in small rodents?

I would like to examine the neuroinvasive properties of tick-borne encephalitis virus in mice and I do not know if macroscopic observation of the brain using Evan's blue is relevant enough to...

09 June 2022 2,430 0 View

Can the electron mobility be estimated with tunneling spectroscopy?

I'm trying to wrap my head around the fact that graphene has a huge electron mobility and a zero density of states (DOS) at the Fermi energy (EF) – yet some other materials can be known as poor...

15 March 2022 697 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

What roles do microbial communities play in the environment and roles played by microorganisms in our environment?

25 July 2024 1,992 2 View

How do bacterial species contribute to ecosystem functions and nutrient cycling and role of microbial communities for sustainability?

25 July 2024 2,351 2 View

How do microbial communities contribute to ecological cycles on Earth and how does an agroecological system support diverse microbial communities?

25 July 2024 5,197 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Can somebody share a diversity and inclusion questionnaire for the business context or military context?

Dear All, Can somebody share a diversity and inclusion questionnaire for the business context or military context. Thank you Anuradha

22 July 2024 1,320 4 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View