I want to stabilize my gelatin structure by EDC/NHS crosslinking so can I just use it with PBS buffer ?

More Pratyush Sarin's questions See All

How to generate topology for a phosphorylated protein?

I have a protein, for which I want to computationally study the effect of its phosphorylation. I was able to add a phosphoryl group to my desired THR residue and the structure looks fine. However,...

21 April 2024 8,607 2 View

Restarting the NAMD Simulation?

Please help me with ideal steps to restart the NAMD simulation from a particular step

29 January 2024 5,285 2 View

How to disperse dry powders of microplastics uniformly on a slide, to be studied under a microscope?

Microplastic polymer is Polyethylene.

19 December 2023 7,158 3 View

Can FRET be done for two proteins outside the cell?

I have read several literatures regarding live cell FRET imaging and quantification over time. But is it possible to do it outside of cell, in vitro? I was recently told that it is possible but...

24 November 2023 5,591 2 View

Is there a software that performs D-NEMD?

Dynamic Non-equilibrium Molecular Dynamics

21 October 2023 7,709 1 View

Can anyone refer me to a simple protocol for Competitive Binding Assay using UV-Vis Absorbance method?

I have two molecules, both peptides: one being a receptor protein and other its viral binding substrate. Both of them have one wild type and 2 mutated variants. I have pretty much figured out how...

19 July 2023 6,981 2 View

Preparation of homogeneous suspension of Polyethylene?

Can anyone suggest a suitable method of preparing a homogeneous suspension of Polyethylene without using a surfactant?

24 May 2023 2,581 1 View

What is the JCPDS No and CCDC no for Ag-MOF-5?

Ag-MOF-5 synthesized from Terepthalic acid, Silver Nitrate and DMF. Need to know Chemical formula, Molecular weight and molecular structure of Ag-MOF-5. Thank you

21 May 2023 1,624 0 View

Can anyone refer me to a good protocol for small peptide sequencing through GCMS?

I am currently trying to characterize a small peptide that was designed computationally and later synthesized and purchased from a manufacturer. I want to perform Gas Chromatography-Mass...

11 May 2023 1,665 3 View

How to process the protein and ligand Isothermal Titration Calorimetry (ITC)?

Hello, I am young researcher working in computational drug discovery and willing to perform experimental work, for which I am planning to start with ITC. Currently, I am reading relevant...

10 March 2023 3,413 3 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How do you obtain a Letter of Access for Citizen Researchers?

Please may I ask if non-salaried Public Contributors conduct data collection interviews or Focus Groups with NHS patients as part of your study? This means that the Public Contributor will either...

29 July 2024 3,085 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

Method to prevent crosstalk fluorescene signal between 2 dye labeled microparticles?

My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....

19 June 2024 2,680 1 View

What adhesive should I use to secure samples that float to the bottom of the well plate?

I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there...

03 June 2024 7,728 2 View

Problem while making hydrogel from chitosan and gelatin ?

A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...

29 May 2024 7,367 4 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View