In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
4,279 views 3,820 posts
Questions related to PCR
Hi, I would like to move serum and cell culture obtained by administering pathogens (bacteria) to mice in a BSL-3 facility to BSL-2 for conducting ELISA experiments. I am aware of various...
13 November 2023 1,378 2 View
I'm exploring the induction of cell lines using TNF-a and would greatly appreciate any guidance on a general protocol or established methods for this process. Has anyone successfully induced cell...
12 November 2023 1,790 0 View
Hi, guys Have you ever extracted RNA from liquor (cerebrospinal fluid) samples? Do you have a protocol to share? In the lab, we normally use the QIAGEN RNeasy kit. Do you think it will work?...
12 November 2023 3,743 4 View
I tested two analytes with the same a.a sequence, just different by 1a.a to one target protein. My SPR outcome looks like the attached figure below. Can I say the right one binds stronger than...
10 November 2023 6,710 2 View
What is an ecological footprint with emphasis on carbon footprint and ecological footprint and carbon footprint concepts relate to global carrying capacity?
03 November 2023 5,271 4 View
The tissue is already mounted on a gelatin coated slide. Is it possible to use a pap pen to provide the barrier for IHC staining?
02 November 2023 6,124 0 View
Very many published papers exist on radiation shielding glasses, all with identical simulation techniques, performed with the MCNPX, FLUKA and GEANT4. I don't really realize if such extensive...
02 November 2023 4,465 6 View
is it due to the immunostaining?
01 November 2023 367 1 View
Hello! I'm working with avian influenza virus, and here we are using the VetMAX-Gold Avian Influenza Virus (AIV) Detection Kit, but I do not know the limit of acid nucleic concentration that the...
30 October 2023 9,288 0 View
Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.
29 October 2023 7,113 1 View
Hello all, I am working with PLJR965 CRISPR plasmid size 8.6kbp. I extracted the plasmid by Alkaline Lysis Method and got 4 bands. Now I am confused that if I have extracted the plasmid...
24 October 2023 9,642 4 View
I use the chemical method of bacterial transformation for pSOUP plasmid. However, I do not have any colonies. Firstly, I used 10 µg of tetracycline, and when it didn’t work, I used 5 µg and still...
24 October 2023 5,914 2 View
why this multiple bands
21 October 2023 9,332 4 View
I am currently working with superpro 9.5 and wish to I put the chemical properties for Carrageenan and Fucoidan for seaweed extraction. The heat capacity modelling of those two substances was done...
21 October 2023 8,440 0 View
I am wondering why there are two recipes to prepare 10X pbs, one for adjust ph value to 7.4, and the other one is to let the ph value remain around 6.8 after solving the salts. The latter one...
20 October 2023 2,919 0 View
Hi, I'm working with environmental DNA metabarcoding techniques to detect fungal biodiversity on soil. It's my first time working with these techniques an dim not trained to see if the PCR looks...
18 October 2023 1,185 0 View
As of now, there is no public database available for this kind of sample to take as a control.
16 October 2023 6,947 3 View
I have a question: Does the sensorgram of the negative response look like the attached figure? I use a CM5 chip immobilised with His to capture my protein and then use other proteins as my ligand....
13 October 2023 6,293 1 View
Which is the best tutorial to learn Next-generation Sequencing online ?
11 October 2023 9,217 2 View
I'm assessing geneflow, relatedness and dispersal patterns in social mole-rats. They live in groups (4-15 animals) with high reproductive skew, 1 reproductive female and 1-2 reproductive males,...
09 October 2023 3,973 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
08 October 2023 1,172 0 View
Now, the burden of sensitive TB forms is decreasing but the role of DR -TB, especially of MDR/XDR - TB forms is increasing.
04 October 2023 3,352 0 View
Hi all, I am currently conducting research that involves the use of PatchDock Unfortunately, I have been unable to obtain the necessary documentation and software from the PatchDock team. If any...
25 September 2023 8,730 2 View
Por favor necesito saber como convertir fuerza de gravedad o sea 16000 g en rpm o sea revoluciones por minuto para trabajar en una centrifuga? Gracias de antemano por su respuesta. Saludos
19 September 2023 7,296 0 View