In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
I want to sequence various strains of Bacillus ssp with the PacBio Multiplex Sequencing Protocol. As you need 1 ug of DNA per strain, we use RotiPinkDNA (linear polyacrylamide with a dye) and...
26 August 2022 3,789 0 View
A thin film solution was prepared at 0.5M concentration and deposited on ITO glass and annealed, the UV analysis revealed transmittance results greater than 100 percent (eg 121%).
13 August 2022 6,603 0 View
I have done a CA Techniques. I didnt have PHA so I was not added in culture setup. Therefore no metaphase was found my sample any possibility is available to get metaphase in sample pls let me know
10 August 2022 367 2 View
I ran the command gmx mdrun -v -deffnm npt_500 -rdd 1 in equilibration step. Then this error came called: Fatal error: There is no domain decomposition for 24 ranks that is compatible with...
05 August 2022 9,345 2 View
Hello, I have done a bit of research to see if there are any cells floating about in the colon (i.e. the lumen part) and cannot find anything that suggests there is, and would like to check if...
28 July 2022 3,018 1 View
I have been using DNASP to run analysis on my sequences, but with linkage disequilibrium the analysis does not run and I get the message in error(2).
22 July 2022 8,489 0 View
Hello! We would like to treat BMDM with the caspase-1 inhibitor Z-WEHD-FMK before bacterial infection. I only found two publications suggesting either 20 or 100 µM for primary macrophages. Of...
07 July 2022 3,931 1 View
In vitro In vivo Methods of extraction and quantification
02 July 2022 8,282 0 View
As stated, I would like to know what is the difference between NCBI blast and EzBioCloud? I have sequences of multiple unknown bacteria and am trying to do the first identification on them...
20 June 2022 7,129 4 View
Hi all, I am struggling to transform my BY4742/1 cells using the Gietz protocol along with a linear PCR fragment. I understand that using a PCR gene disruption cassette is not as efficient as...
15 June 2022 7,214 3 View
Is it necessary to be in empty stomach of fasting( drinks) for at least 8-12 hours before lower abdomen USG? Or eating and drinking is allowed? why pressure in Urinary bladder (urination)is...
11 June 2022 8,172 10 View
I saw this structures on my primary culture of mesenchymal cells but idk what are those, does anybody knows what are those things?
29 May 2022 1,068 3 View
Hi. Can Anyone provide me with sample papers using mantel-hanzel chi square test to control for confounding? Need to see the example of how the result being reported. Thanks
23 May 2022 8,638 0 View
Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks
20 May 2022 1,207 3 View
I want to expose my lung epithelial cells to different treatments in vitro and then detect pro- and anti-inflammatory cytokines/chemokines in the conditioned media - can I then do PCRs on the...
19 May 2022 2,656 4 View
I found an exon skipping event by RT-PCR I want to assess whether exon skipping changes the reading frame? And is this variant translatable? Is there any method or software to detect it?
19 May 2022 9,457 2 View
Recently, we have done the macrophage antigen lpresenting experiment by co-culture of macrophages pre-loaded with OVA and CD4+/CD8+ T cells isolated from OT-II/OT-I mouse spleen. Using CFSE to...
11 May 2022 1,727 6 View
Hello, I'm trying to clone my amplified insert in pGEM-T vector using T4 DNA ligase and not getting positive clones even after trying variations in vector to insert ratio. Amplification of insert...
22 April 2022 975 2 View
I picked a primer sequence ,designed by the author, for my PCR from a published paper. The issue is: UCSC Insilico Pcr is not browsing that sequence, although a detailed study is done on these...
18 April 2022 8,125 6 View
I have outsourced Sanger sequencing of my purified PCR products. Looking at the chromatogram, I know to trim the ends. However, the ends that need to be trimmed according to the quality scores are...
08 April 2022 7,574 3 View
I want to extract DNA from buccal swabs using proteinase K and the amount of DNA should be in higher concentration, PCR process.
07 April 2022 2,162 3 View
Why is urinary exosomes isolated at 200000 g whereas at 120000 g for cell derived exosomes as mentioned in several papers. Will not these two different speeds yield different vesicles of...
03 April 2022 9,672 0 View
Each taken gene ontology for dysregulated genes set in colorectal cancer has a biological function in neurogenesis. Can anyone explain why?
21 March 2022 3,010 3 View
As I am trying to dock protein with an organometallic ligand in autodock Vina, I'm getting some errors. So can someone guide me on how to assign a partial charge to a metal center and how to dock...
13 March 2022 5,127 1 View