In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
Hi, I am having issue with the ladder peak detection. I could not find where to manually modify peak settings. The error message is "Not all expected ladder peaks have been found. You should try...
31 May 2023 9,858 0 View
I made my ligations using pGEM-T easy vector system I, I normally used PCR products (product for TA cloning-Taq DNA polymerase and Control Insert DNA (promega like a ligation control) for the...
26 May 2023 3,589 4 View
How can we analyze the Sanger sequencing result for detecting indels or substitutions? Is pair-wise alignment enough for finding out indels or is any particular software available for finding the...
29 April 2023 3,503 3 View
Are the binding constant and binding affinity is the same thing in both fluorescence quenching or fluorescence increment? What is the meaning of getting high or low binding constant (kB), derived...
28 April 2023 7,686 2 View
What are some common pests and diseases that affect wheat?
17 April 2023 6,831 2 View
I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...
10 March 2023 4,933 2 View
How can you downloaded SNPs from NCBI after the last update on the site?
21 February 2023 4,090 2 View
Thank you for your suggestions.
01 February 2023 7,902 2 View
Hi, I am searching for a SPECIFIC myofibroblast and fibroblast marker for human lung tissue to identify fibroblasts in different lung diseases (IPF, carcinoma, OP etc.). I already tried CD90, TE-7...
17 January 2023 6,331 0 View
Democracy and Consensus in African Traditional politics: A Plea for a non-party?? I need the full text
16 January 2023 8,547 2 View
I use ENMeval 2 and confused about including "kfolds =" in the ENMevaluate function settings. Could anyone advise what I am doing wrong in the string below? e.mxjar
31 December 2022 1,724 0 View
Hi everyone, I have a SLM and It seems to be dead. When I open the controlling software and try to change the phase of the SLM, there is no response back to our software. I tried to restart it few...
21 December 2022 5,113 3 View
I'm working with microsatellite (SSR) and get different allele sizes from the same primer. The difference in alleles ranges from 1 - 4 bp. For example, in the first experiment I got alleles of...
05 December 2022 2,568 2 View
Hello! I need some help troubleshooting. I have attached a merged image below and I am wondering why my merge channels look pink but my red and blue channels look a nice red and a nice blue. Why...
22 November 2022 4,088 3 View
I would love for this code to generate a graphy at the end.Please assist
15 November 2022 6,735 2 View
Hi, Well, epitopes that bind with MHC are considered antigenic. my question is what is the need to predict antigenicity or allergenicity? I mean if we have predicted epitope data and we know...
07 October 2022 1,596 2 View
SInce Notch is a cell signalling pathway, and yeast is unicellular, does notch exist in yeast?
02 October 2022 3,204 3 View
How to Assemble ITS 1 and ITS 2 Sequence for Isolate submission to ncbi ? I have already tried submitting by finding the similarity of my test fungal ITS Sequences with the ncbi blast, where...
19 September 2022 7,904 1 View
I want to measure antioxidant enzyme levels of SOD, CAT and GSH the problem is that the manuals from the manfacture are too difficult to understand
19 September 2022 9,617 9 View
I have experimented with alternative splicing in Exon 11 BRCA1, and I need to know how I can provide analysis and count my bp. We used two methods for gel, one regular and one automated by Qiagen...
12 September 2022 1,743 0 View
I need to do RNA extractions of a large number of samples from developing wheat grain, and plan to do them in the batches of 12. Is is sensible to keep the homogenised samples (with pestle and...
06 September 2022 7,872 1 View
Will really appreciate for any suggestions (references with synthetic procedure descriptions) on synthesis of fully substituted p-tertbutylcalix[4]arenes stabilized exceptionally in "cone"...
06 September 2022 7,479 0 View
I use CRC organoids to detect the protein expression of β-tubulin, but why can't it be detected on day 7? Logically, the organoids have already formed on the seventh day.
06 September 2022 4,209 0 View
I know that a T7 promoter can be added to the 5' end of the primers in PCR and RPA. I'm wondering if the same is also true for HDA or is there a way to get RNA products from a HDA reaction? (Or...
05 September 2022 1,104 0 View