In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
I noticed that a lot of plasmid annotations tend to find ColE1 origins, but I can never usually find the b. subtilis origin of replications from annotations.
22 March 2024 7,292 2 View
Hi, I did FTIR of my oxidized biochar samples and found that peak area is larger after deloading of phosphorus rather than loading of phosphorus. So far i know peak area will be smaller after...
16 March 2024 5,182 1 View
Procedure for constructing a two-dimensional scatter plot in the Statistical Package for the Social Sciences (SPSS), incorporating a mean trace line, with one nominal variable and two...
04 March 2024 6,397 0 View
JAS Pi.ai LLM (Assimilation: Copy & Paste) describe: quasi-homuncular Embodied Semantic Models (ESMs) Quasi-homuncular Embodied Semantic Models (ESMs) are a theoretical concept that combines...
03 March 2024 3,239 2 View
Our lab buys 500 mL bottles of RPMI-1640 Medium and stores them at 4C. Oftentimes, for my experiments, I only need about 100 mL total. So, I will make RPMI-1640 + 10% FBS with 90 mL of media and...
23 February 2024 4,597 2 View
I encountered the fluorescence enhancement in the DNA interaction process. Since the classical Stern-Volmer equation is used for the fluorescence quenching process, this equation cannot be used to...
17 February 2024 2,840 1 View
I have faced this problem with different types of tissues whenever i immerse them in formaldehyde and then embed them in OCT (I always pat them dry before embedding). When I section them, I get...
12 February 2024 4,478 0 View
Can a black hole destroy matter and how does nitrogen get from the atmosphere to the biosphere?
25 January 2024 5,787 2 View
I am writing to request permission to utilize your tools in my Ph.D. thesis on the knowledge, attitude, and behavior of registered nurses toward pressure injury prevention. Your expertise in this...
20 January 2024 8,735 0 View
The cells are genetically engineered and grown on PDL plates for a potency assay. My BCA is highly variable. I am wondering if triton disrupts the PDL coating, which then interferes in the BCA...
11 January 2024 8,637 1 View
Is it possible to identify statistical differences between two groups when they have different sample sizes, as in the case of comparing CD (with 12 samples) to an experimental group (with 2 samples)?
27 December 2023 9,898 3 View
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20 December 2023 4,140 3 View
19 December 2023 9,493 3 View
19 December 2023 6,272 4 View
Hi there. I want to resample all Sentinel-2 bands to 10 meters. I know snappy has several methods for this, but I was wondering what approaches or packages in python outside of Snappy do you think...
18 December 2023 2,250 1 View
I'm trying to find research on wildlife population estimation of a species in which individuals cannot be differentiated from each other. We have used Mark-recapture to estimate population size,...
17 December 2023 4,185 7 View
This question refers to the theory of functions of complex variable.
10 December 2023 9,208 1 View
Brain and other neural degenerative disorders are less common in human female during adolescent as compare to males?
08 December 2023 2,519 2 View
I need to enrich for mitotic cells for immunofluorescence. Since the mitotic cells are rounded I need to increase the adherence to the surface of the plate. So far I have been using only...
29 November 2023 9,639 0 View
What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal, and how does this information contribute to the...
27 November 2023 3,876 0 View
We used to use NADPH as the gene used for nornalization in rat liver experiment. However, NADHP ( and beta actin as well?) may be altered in the setting of fatty liver related experiment. We use...
26 November 2023 2,050 1 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
22 November 2023 4,310 0 View
Dear all, I am trying to differentiate human pulmonary fibroblast into myofibroblast using human recombinant TGFB1 (10ng/ml) with 48 hrs incubation. For my experiments so far, I could not see a...
14 November 2023 7,063 1 View
The link listened in the original paper published for downloading this software does not work anymore.
14 November 2023 5,347 0 View