In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
I use the chemical method of bacterial transformation for pSOUP plasmid. However, I do not have any colonies. Firstly, I used 10 µg of tetracycline, and when it didn’t work, I used 5 µg and still...
24 October 2023 5,848 2 View
why this multiple bands
21 October 2023 9,232 4 View
I am currently working with superpro 9.5 and wish to I put the chemical properties for Carrageenan and Fucoidan for seaweed extraction. The heat capacity modelling of those two substances was done...
21 October 2023 8,391 0 View
I am wondering why there are two recipes to prepare 10X pbs, one for adjust ph value to 7.4, and the other one is to let the ph value remain around 6.8 after solving the salts. The latter one...
20 October 2023 2,839 0 View
Hi, I'm working with environmental DNA metabarcoding techniques to detect fungal biodiversity on soil. It's my first time working with these techniques an dim not trained to see if the PCR looks...
18 October 2023 1,108 0 View
As of now, there is no public database available for this kind of sample to take as a control.
16 October 2023 6,882 3 View
I have a question: Does the sensorgram of the negative response look like the attached figure? I use a CM5 chip immobilised with His to capture my protein and then use other proteins as my ligand....
13 October 2023 6,224 1 View
Which is the best tutorial to learn Next-generation Sequencing online ?
11 October 2023 9,159 2 View
I'm assessing geneflow, relatedness and dispersal patterns in social mole-rats. They live in groups (4-15 animals) with high reproductive skew, 1 reproductive female and 1-2 reproductive males,...
09 October 2023 3,864 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
08 October 2023 1,102 0 View
Now, the burden of sensitive TB forms is decreasing but the role of DR -TB, especially of MDR/XDR - TB forms is increasing.
04 October 2023 3,240 0 View
Hi all, I am currently conducting research that involves the use of PatchDock Unfortunately, I have been unable to obtain the necessary documentation and software from the PatchDock team. If any...
25 September 2023 8,658 2 View
Por favor necesito saber como convertir fuerza de gravedad o sea 16000 g en rpm o sea revoluciones por minuto para trabajar en una centrifuga? Gracias de antemano por su respuesta. Saludos
19 September 2023 7,212 0 View
Effect of Microplastics on the Germination and Growth of Mangrove Plants
18 September 2023 9,338 0 View
I have a high number of bacterial cultures I am growing, extracting the DNA from, and sequencing as short reads. Due to the format I'm using (96-well plates), cross-sample contamination is a big...
04 September 2023 4,426 3 View
I tried amplifying some plant DNA I extracted with 5 types of primers. In some DNA samples, I observe electrophoresis gel bands just with one PCR procedure. But some samples do not show gel bands...
04 September 2023 5,011 0 View
I am in the process of identifying specific small RNAs from raw data obtained through total small RNA sequencing. Could you guide me on aligning these particular small RNAs with the gene sequence...
24 August 2023 1,756 0 View
While looking for my protein of interest, I came across PDB structures obtained from uncultured bacterium. How are these crystal structures different and what is the credibility of using these...
24 August 2023 5,280 0 View
I wish to know that what concentration of cDNA(in ng) will be efficient for the Real time PCR. If Master mix will be SYBR green. Please provides us appropriates answer. Thanks in Advance.
07 August 2023 5,407 2 View
I hope to know the interpretation of this results. Thanks
28 July 2023 7,439 0 View
I have 30 days old spheroids (organoids) which originate from stromal vascular fraction. They are usually big in size, growing in 96-wells. I can use TrypLE, Accutase, Coagenase, Trypsin, or...
26 July 2023 6,614 1 View
I performed an umbrella sampling using as CV the rotation angle of protein helix. But I didn’t use pulling md to produce configurations. I used enforced rotation to generate the configurations....
17 July 2023 6,316 0 View
Hello, I want to grow bacteria on glass coverslips by immersing them in a liquid bacterial culture. I would like to fix them to the bottom of petri plates or well plates , because otherwise they...
16 June 2023 6,212 4 View
Are there any online platforms to find palindromic sequences in a promoter region of a gene that might be targets for a transcriptional regulator protein?
05 June 2023 8,532 4 View