RNA extraction from liquor samples??

More Isabela Fonseca's questions See All

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

Hello! Can anyone help identify this isopod?

is deep sea, about 3000 m

17 April 2024 2,087 0 View

What criteria ensure a training system boosts communication & planning efficiency for diverse roles & shifts in port operations?

In my research on developing an integrated training management system for industrial port complexes, I'm investigating the role of automated notification systems. This is crucial because these...

09 April 2024 1,348 0 View

¿How do I measure the angle of a Closterium spp?

Is it measured from the apical parts to the center of the microalgae ? or do you have another ways to measured it? I attach an image to explain thanks

12 February 2024 6,923 3 View

How to determine IC50 significance?

Hi guys, I am performing MTT assays to charactherize tumor cells resistance to chemotherapy (I am comparing the treated cells vs. control (non treated) from different tumor cell lines). I have...

31 January 2024 2,622 4 View

How can I solve this problem?

When I tried to prepare a solution of enzalutamide of 1000 uM in cell culture medium from a solution of 1 M of enzalutamide in DMSO the drug precipitated. I've tried changing the pH and the...

08 December 2023 3,946 1 View

PTFE as a binder for Lithium ion batteries cathode, how to ?

Numerous articles mention the combination of metal oxide, carbon black, and PTFE as a binder, followed by pressing onto an Al mesh. Yet, I encounter difficulties in achieving uniform pellets using...

07 December 2023 4,297 3 View

What are some improved protocol for RNA extraction from fish testis and semen?

I have tried to extract RNA from fish semen and testis using the RNEasy kit and also have the PureLink RNA kit. I have tried a few modifications I've found online, but the RNA comes either highly...

06 September 2023 7,663 2 View

How can I accurately calculate the NP Ratio for Li-Ion Batteries, and what's the essential surface area to consider?

Hello, In numerous research papers, they mention N/P ratios, measured in mA/cm2 units. I am curious about the specific area considered in a coin cell. When envisioning the cathode as a cylinder,...

24 July 2023 958 1 View

Lithium ion battery cycling voltages, how to choose ?

Hi all, In electrochemical measurements, it is intriguing to me how different voltage windows are elected for various active materials. Could someone please cite the key parameters to bear in...

11 July 2023 8,396 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,881 2 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,513 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,705 0 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

Robert Adolf Brinzer

Can you please specify the type of liquor you are wanting to extract from? Are you referring to black liquor from paper processing?

Péter Gyarmati

I have never, but this may be the closest:

https://www.ajevonline.org/content/62/1/99

Arti Sharma

I don't know about the liquor but you can try TRIZOL method for extraction of RNA.

Isabela Fonseca

Hi! Thank you all for your answers. I am talking about RNA extraction from cerebrospinal fluid samples.