What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

What is the appropriate period for indexing an article in the Scopus database?

Dear RG members The indexing of a published article in the Scopus database may depend on several factors. The issue is that my colleague and I have published separately two articles in the same...

16 December 2020 4,361 19 View

I need equations to calculate the activity of the enzyme glutamanase and basic phosphatase in soil and plants?

I need the rates for calculating the mentioned enzymes after the laboratory work. I have trouble calculating and extracting the activity of the mentioned enzymes in soil and plants because of the...

03 November 2020 8,601 1 View

How can I know that a variant is novel?

I found a variant which is not reported in gnomAD or ExAC, but still has an rs. Should I consider it as a novel variant? when can I say that ceratin variant is novel?

15 October 2020 9,539 3 View

Do you have any qualitative or quantitative methodologies for evaluating gender equality plans in HE?

I am collating evaluation methodologies. So, broader methodologies for inclusion in my report are also welcome. Please also indicate the preferred citation for the evaluation methodology.

14 October 2020 4,266 7 View

How to compare the effect of changing single amino acid in a protein in a 3D model structure using Swiss-Model?

I run sequening on a sample and found a pathogenic variant. Then I draw a 3Dprotein model on Swiss-Model for the wild type protein and the mutatnt protein. I don't know how to comment on things...

05 October 2020 579 4 View

What are the recent research direction of Skin Cancer Detection ?

I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research...

04 October 2020 8,628 1 View

What can I refer for Depth estimation using multiple camera input?

Currently, I am working on my final year project which involves depth estimation form multiple camera inputs for video analysis purposes. I am new in this field and struggling to look a the...

30 September 2020 3,642 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View