In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
Is it possible to identify statistical differences between two groups when they have different sample sizes, as in the case of comparing CD (with 12 samples) to an experimental group (with 2 samples)?
27 December 2023 9,797 3 View
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20 December 2023 4,065 3 View
19 December 2023 9,405 3 View
19 December 2023 6,186 4 View
Hi there. I want to resample all Sentinel-2 bands to 10 meters. I know snappy has several methods for this, but I was wondering what approaches or packages in python outside of Snappy do you think...
18 December 2023 2,153 1 View
I'm trying to find research on wildlife population estimation of a species in which individuals cannot be differentiated from each other. We have used Mark-recapture to estimate population size,...
17 December 2023 4,102 7 View
This question refers to the theory of functions of complex variable.
10 December 2023 9,126 1 View
Brain and other neural degenerative disorders are less common in human female during adolescent as compare to males?
08 December 2023 2,467 2 View
I need to enrich for mitotic cells for immunofluorescence. Since the mitotic cells are rounded I need to increase the adherence to the surface of the plate. So far I have been using only...
29 November 2023 9,584 0 View
What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal, and how does this information contribute to the...
27 November 2023 3,673 0 View
We used to use NADPH as the gene used for nornalization in rat liver experiment. However, NADHP ( and beta actin as well?) may be altered in the setting of fatty liver related experiment. We use...
26 November 2023 1,970 1 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
22 November 2023 4,228 0 View
Dear all, I am trying to differentiate human pulmonary fibroblast into myofibroblast using human recombinant TGFB1 (10ng/ml) with 48 hrs incubation. For my experiments so far, I could not see a...
14 November 2023 6,980 1 View
The link listened in the original paper published for downloading this software does not work anymore.
14 November 2023 5,283 0 View
Hi, I would like to move serum and cell culture obtained by administering pathogens (bacteria) to mice in a BSL-3 facility to BSL-2 for conducting ELISA experiments. I am aware of various...
13 November 2023 1,291 2 View
I'm exploring the induction of cell lines using TNF-a and would greatly appreciate any guidance on a general protocol or established methods for this process. Has anyone successfully induced cell...
12 November 2023 1,693 0 View
Hi, guys Have you ever extracted RNA from liquor (cerebrospinal fluid) samples? Do you have a protocol to share? In the lab, we normally use the QIAGEN RNeasy kit. Do you think it will work?...
12 November 2023 3,643 4 View
I tested two analytes with the same a.a sequence, just different by 1a.a to one target protein. My SPR outcome looks like the attached figure below. Can I say the right one binds stronger than...
10 November 2023 6,492 2 View
What is an ecological footprint with emphasis on carbon footprint and ecological footprint and carbon footprint concepts relate to global carrying capacity?
03 November 2023 5,158 4 View
The tissue is already mounted on a gelatin coated slide. Is it possible to use a pap pen to provide the barrier for IHC staining?
02 November 2023 6,028 0 View
Very many published papers exist on radiation shielding glasses, all with identical simulation techniques, performed with the MCNPX, FLUKA and GEANT4. I don't really realize if such extensive...
02 November 2023 4,385 6 View
is it due to the immunostaining?
01 November 2023 286 1 View
Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.
29 October 2023 7,032 1 View
Hello all, I am working with PLJR965 CRISPR plasmid size 8.6kbp. I extracted the plasmid by Alkaline Lysis Method and got 4 bands. Now I am confused that if I have extracted the plasmid...
24 October 2023 9,557 4 View