In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
if samples are limited, e.g., less than 10 samples per population, which molecular markers are suitable(Microsatellites or SNPs generated by RAD-seq)? If you can provide some references for your...
05 March 2022 2,081 4 View
How can groundwater affect forest Restoration/ aforestation?
05 March 2022 5,502 11 View
i am working on research project in which i am applying normal irrigation to tomato plants in pots experiment after three days. i have selected two other irrigation intervals 6 and 9 days. if i...
01 March 2022 1,554 3 View
I am trying to run BBmap but I am getting this error ~/bbmap$ bash stats.sh /root@wch7p10204:/home/szweda/bbmap/resources/phix174_ill.ref.fa.gz Exception in thread "main"...
09 February 2022 5,780 2 View
Hello, I have been working on using qPCR as part of a project to determine relative telomere length in birds. However, my r2 and efficiencies have been inconsistent, sometimes returning...
01 February 2022 2,571 1 View
I have 8 sequences of the enzyme creatine kinase from organism A. I am interested in obtaining a consensus sequence to later use it as a template to search for this sequence in the genome of...
01 February 2022 3,156 1 View
Hello all, I have been working on in-silico analysis of plant TFs. I found that there is a numbering after each TF like OsWRKY1, TaDREB15, etc. I am not able to find out how to assign these...
31 January 2022 2,664 4 View
Why is genomic DNA used in cloning vectors of kinetoplastids? When in eukaryotes you use cDNA from mRNA?
25 January 2022 620 2 View
I bought a synthetic gene from a vendor, the gene is ligated in the pUC57 plasmid (with ampicilin resistance). the pUC57 plasmid have been tested using electrophoresis and it's all good. I'm...
11 January 2022 1,557 18 View
I am looking for customized tetramer synthesis for HLA DQ epitopes (MHC class-II), so could you please suggest sources please.
29 December 2021 5,668 0 View
Hi, I have some left over spin columns from my gel extraction kit. I was wondering if I could use this with my RNA extraction buffer to extract RNA? Has anyone tried this?
14 December 2021 5,807 4 View
Hi everyone, I was trying to check the RT-PCR product which is less than 100bp on the agarose gel. I used 3% agarose and ran 80V for 1.5 hr, then I put my gel in the 1XTAE buffer with GelRED to...
10 December 2021 9,775 3 View
I'm trying to clone some cDNA, but when I PCR the cDNA with my primers, I get no product on the gel. So I checked around and found out that I might be missing the second strand synthesis step,...
09 December 2021 4,326 3 View
I will also further do the restriction digestion
06 December 2021 4,874 10 View
Dear all, I am cloning N-terminal GFP with Linker-MCS exchanging with C-terminal GFP in PBCAG-eGFP (addgene 40973) vector, the enzymes I used are EcoRI/NotI. This sounds like a very simple...
03 December 2021 1,254 4 View
I work in an industry so can't use Addgene.
02 December 2021 8,330 2 View
16s rRNA gene sequencing/metagenomics.
29 November 2021 7,297 9 View
As I have started to learn primer designing, I have come across terms like restriction sites, sitting sequences, etc. I wanted to know if sitting sequences added before the restriction sites are...
26 November 2021 1,597 3 View
For example if I am comparing Rt PCR ct value with the RDT test positive or negative for Covid 19.
26 November 2021 4,079 3 View
Hi, I started using Lightcycler 480 for relative quantification and I couldn't find a way to get basic cp-values for each well on the plate from the Lightcycler 480 program. I want to calculate...
21 November 2021 4,534 0 View
Hello, I am working on a hybridoma cloning and sequencing experiment. I did RT PCR to produce cDNA followed by amplification on PCR. Then i purified the gel and extracted DNA from the samples....
18 November 2021 7,414 3 View
Hello everyone, I need your recommendation about the best method to detect variants for a specific gene in a big sample. let us say that I wanted to detect variants of CYP2C19 in 100 patients,...
18 November 2021 6,576 3 View
I am working with malaria-infected blood samples. Currently I am using PCR to determine allele frequencies of my genes of interest. I would like to determine the specific antibody responses (IgG)...
09 November 2021 1,464 2 View
Hi, I have obtained putative transgenic Nipponbare plants by 3 successive selection on antibiotic media. But I am getting negative results for PCR whereas, I got amplification in housekeeping gene...
09 November 2021 1,965 3 View