In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
Effect of Microplastics on the Germination and Growth of Mangrove Plants
18 September 2023 9,422 0 View
I have a high number of bacterial cultures I am growing, extracting the DNA from, and sequencing as short reads. Due to the format I'm using (96-well plates), cross-sample contamination is a big...
04 September 2023 4,486 3 View
I tried amplifying some plant DNA I extracted with 5 types of primers. In some DNA samples, I observe electrophoresis gel bands just with one PCR procedure. But some samples do not show gel bands...
04 September 2023 5,056 0 View
I am in the process of identifying specific small RNAs from raw data obtained through total small RNA sequencing. Could you guide me on aligning these particular small RNAs with the gene sequence...
24 August 2023 1,837 0 View
While looking for my protein of interest, I came across PDB structures obtained from uncultured bacterium. How are these crystal structures different and what is the credibility of using these...
24 August 2023 5,361 0 View
I wish to know that what concentration of cDNA(in ng) will be efficient for the Real time PCR. If Master mix will be SYBR green. Please provides us appropriates answer. Thanks in Advance.
07 August 2023 5,461 2 View
I hope to know the interpretation of this results. Thanks
28 July 2023 7,495 0 View
I have 30 days old spheroids (organoids) which originate from stromal vascular fraction. They are usually big in size, growing in 96-wells. I can use TrypLE, Accutase, Coagenase, Trypsin, or...
26 July 2023 6,691 1 View
I performed an umbrella sampling using as CV the rotation angle of protein helix. But I didn’t use pulling md to produce configurations. I used enforced rotation to generate the configurations....
17 July 2023 6,398 0 View
Hello, I want to grow bacteria on glass coverslips by immersing them in a liquid bacterial culture. I would like to fix them to the bottom of petri plates or well plates , because otherwise they...
16 June 2023 6,281 4 View
Refer Chi-Square Tests, Goodness of Fit Test Problem -> https://youtu.be/5L4balz_bp8
16 June 2023 2,709 3 View
Are there any online platforms to find palindromic sequences in a promoter region of a gene that might be targets for a transcriptional regulator protein?
05 June 2023 8,650 4 View
Hi, I am having issue with the ladder peak detection. I could not find where to manually modify peak settings. The error message is "Not all expected ladder peaks have been found. You should try...
31 May 2023 9,920 0 View
I made my ligations using pGEM-T easy vector system I, I normally used PCR products (product for TA cloning-Taq DNA polymerase and Control Insert DNA (promega like a ligation control) for the...
26 May 2023 3,643 4 View
How can we analyze the Sanger sequencing result for detecting indels or substitutions? Is pair-wise alignment enough for finding out indels or is any particular software available for finding the...
29 April 2023 3,721 3 View
Are the binding constant and binding affinity is the same thing in both fluorescence quenching or fluorescence increment? What is the meaning of getting high or low binding constant (kB), derived...
28 April 2023 7,737 2 View
What are some common pests and diseases that affect wheat?
17 April 2023 6,912 2 View
The topic of my research is to investigate the quality of life for postnatal women with urinary incontinence. Most of the articles I read were from other countries and not postnatal women alone....
13 March 2023 5,833 0 View
I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...
10 March 2023 5,027 2 View
In a case with a patient developing a pseudobulbar syndrome, will we have to think of another diagnosis in case of presenting urinary incontinence?
23 February 2023 2,046 0 View
How can you downloaded SNPs from NCBI after the last update on the site?
21 February 2023 4,137 2 View
Thank you for your suggestions.
01 February 2023 7,970 2 View
Hi, I am searching for a SPECIFIC myofibroblast and fibroblast marker for human lung tissue to identify fibroblasts in different lung diseases (IPF, carcinoma, OP etc.). I already tried CD90, TE-7...
17 January 2023 6,405 0 View
Democracy and Consensus in African Traditional politics: A Plea for a non-party?? I need the full text
16 January 2023 8,601 2 View