I'm assessing geneflow, relatedness and dispersal patterns in social mole-rats. They live in groups (4-15 animals) with high reproductive skew, 1 reproductive female and 1-2 reproductive males, with overlapping generations. The other group members are usually offspring of the breeders and don't contribute to breeding. Some unrelated immigrants do occur. I'm using SPAGeDI to estimate dispersal distances. Now some papers recommend removing offspring in genetic analyses so that you don't include pre-dispersal individuals. So I did that for Fst, relatedness coeff, and isolation by distance. For dispersal distances initially I thought to use just the reproductive individuals, but my sample size for females is 32 so I used all adults regardless of reproductive status getting me 84 males and 97 females. I used a population density equal to the expected number of breeding individuals per group in my study area, not the total number of individuals I captured. The values for males did not converge (no matter what density I used), and the predicted neighborhood size for females is 3 times than what I observed through captures. The predicted neighborhood size for both sexes combined is fairly accurate.

So a few questions: 1) when estimating dispersal distances through genetic methods do I limit my dataset to just reproductive individuals or use all the individuals even if there is overlapping generations? 2) specifically for SPAGeDi the population density you specify, is it the number of breeders per sq km or the total number of individuals (census density)? 3) assuming i used an accurate population density for the adult females, is the neighborhood size accurate? Thank you in advance