In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
I use ENMeval 2 and confused about including "kfolds =" in the ENMevaluate function settings. Could anyone advise what I am doing wrong in the string below? e.mxjar
31 December 2022 1,799 0 View
Hi everyone, I have a SLM and It seems to be dead. When I open the controlling software and try to change the phase of the SLM, there is no response back to our software. I tried to restart it few...
21 December 2022 5,192 3 View
I saw Thermal emission script in in optics.ansy.com but there is some problems, in my simulation I need have diagram for 4 different radius and period. I don't know how to change script for that...
07 December 2022 9,533 0 View
I'm working with microsatellite (SSR) and get different allele sizes from the same primer. The difference in alleles ranges from 1 - 4 bp. For example, in the first experiment I got alleles of...
05 December 2022 2,653 2 View
Hello! I need some help troubleshooting. I have attached a merged image below and I am wondering why my merge channels look pink but my red and blue channels look a nice red and a nice blue. Why...
22 November 2022 4,168 3 View
I would love for this code to generate a graphy at the end.Please assist
15 November 2022 6,795 2 View
Hi, Well, epitopes that bind with MHC are considered antigenic. my question is what is the need to predict antigenicity or allergenicity? I mean if we have predicted epitope data and we know...
07 October 2022 1,665 2 View
SInce Notch is a cell signalling pathway, and yeast is unicellular, does notch exist in yeast?
02 October 2022 3,273 3 View
How to Assemble ITS 1 and ITS 2 Sequence for Isolate submission to ncbi ? I have already tried submitting by finding the similarity of my test fungal ITS Sequences with the ncbi blast, where...
19 September 2022 7,992 1 View
I want to measure antioxidant enzyme levels of SOD, CAT and GSH the problem is that the manuals from the manfacture are too difficult to understand
19 September 2022 9,739 9 View
I have experimented with alternative splicing in Exon 11 BRCA1, and I need to know how I can provide analysis and count my bp. We used two methods for gel, one regular and one automated by Qiagen...
12 September 2022 1,833 0 View
I need to do RNA extractions of a large number of samples from developing wheat grain, and plan to do them in the batches of 12. Is is sensible to keep the homogenised samples (with pestle and...
06 September 2022 7,969 1 View
Will really appreciate for any suggestions (references with synthetic procedure descriptions) on synthesis of fully substituted p-tertbutylcalix[4]arenes stabilized exceptionally in "cone"...
06 September 2022 7,562 0 View
I use CRC organoids to detect the protein expression of β-tubulin, but why can't it be detected on day 7? Logically, the organoids have already formed on the seventh day.
06 September 2022 4,266 0 View
I know that a T7 promoter can be added to the 5' end of the primers in PCR and RPA. I'm wondering if the same is also true for HDA or is there a way to get RNA products from a HDA reaction? (Or...
05 September 2022 1,178 0 View
I want to sequence various strains of Bacillus ssp with the PacBio Multiplex Sequencing Protocol. As you need 1 ug of DNA per strain, we use RotiPinkDNA (linear polyacrylamide with a dye) and...
26 August 2022 3,878 0 View
A thin film solution was prepared at 0.5M concentration and deposited on ITO glass and annealed, the UV analysis revealed transmittance results greater than 100 percent (eg 121%).
13 August 2022 6,675 0 View
I have done a CA Techniques. I didnt have PHA so I was not added in culture setup. Therefore no metaphase was found my sample any possibility is available to get metaphase in sample pls let me know
10 August 2022 468 2 View
I ran the command gmx mdrun -v -deffnm npt_500 -rdd 1 in equilibration step. Then this error came called: Fatal error: There is no domain decomposition for 24 ranks that is compatible with...
05 August 2022 9,469 2 View
Hello, I have done a bit of research to see if there are any cells floating about in the colon (i.e. the lumen part) and cannot find anything that suggests there is, and would like to check if...
28 July 2022 3,106 1 View
I have been using DNASP to run analysis on my sequences, but with linkage disequilibrium the analysis does not run and I get the message in error(2).
22 July 2022 8,579 0 View
I am interested in measuring the signalling activity of induced pluripotent stem cell-derived cortical interneurons in vitro. The interneuron population would be diverse and include common types...
20 July 2022 4,083 3 View
Hello! We would like to treat BMDM with the caspase-1 inhibitor Z-WEHD-FMK before bacterial infection. I only found two publications suggesting either 20 or 100 µM for primary macrophages. Of...
07 July 2022 4,020 1 View
In vitro In vivo Methods of extraction and quantification
02 July 2022 8,356 0 View