Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.
Malcolm Nobre
Hello Aleena Varughese
You should follow the TRIzol method for RNA isolation from cells. I have provided a detailed protocol in the link below.
https://www.researchgate.net/post/How_can_I_perform_Trizol_RNA_extraction_from_adherent_cell_line/1
If you are using suspension cells you may follow these steps:
a. Pellet the cells by centrifugation and discard the supernatant.
b. Add 0.75 mL of TRIzol reagent (per 5– 10 × 10^6 cells) to the pellet.
c. Pipet the lysate up and down several times to homogenize.
You may then continue from step 6 from the above protocol provided in the link.
Good Luck!
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