How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How i can Improve the accuracy of an artificial intelligence model for test data in MATLAB ?

Hello everyone, I have a question about artificial intelligence in MATLAB. Can you help me, to improve the accuracy of an artificial intelligence model for test data in MATLAB software? (while...

22 July 2020 2,692 12 View

Does anyone know how can I get Mito Thermo Yellow (MTY) dye?

This is a thermosensitive dye that was recently used by chretien et al to measure changes in mitochondrial temperatures.

01 March 2020 4,863 4 View

If a staph bacteria shows resistance to vancomycin disk on Mueller Hinton plates, does it mean its Methecillin resistance?

Also can staph aureus bacteria appear pink on Manitol salt agar plates? Or it definitely appears yellow? Has anyone isolated staph aureus with alpha hemolytic characteristics? Thanks.

06 February 2019 409 4 View

What kind of emulsifier you propose for emulsion-based drinks with acidic pH having long shelf life?

Beside lecithin, some kind of sucrose ester FA, whey protein, do you know some kind of emulsifiers that are able to produce a long-term stable O/W emulsion ?

18 December 2018 2,759 4 View

How to use yalom existential psychotherapy or group therapy in school setting?

Psychotherapy can help most patients confront the issue of responsibility for their own life, problems, and improvement (Yalom, 1974). Group therapy can be somewhat beneficial for clients who are...

12 December 2018 6,850 3 View

What are the safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix?

Hi for All I need your recommendations from safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix. Thanks in advance

06 July 2018 326 2 View

Do you know the latest list of the journals that discontinued from Scopus?

Hi dear colleagues Do you know the latest list of the journals that discontinued from Scopus? You can see it in this file which downloaded from Scopus website (last updated 26/3/2018). Regards

14 April 2018 4,977 17 View

From the copyright viewpoint, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform?

Hi dear colleagues. From the copyright viewpoint for journals, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform? This question...

05 April 2018 9,253 74 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View