In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
Hi there, I am aware that there are lots of questions about applying t test to qPCR results. But I need a clear answer. I have my Delta Delta Ct fold change results as in the supplementary file....
23 September 2021 655 1 View
Like if I amplify a specific gene fragment of a Ricketessia strain, Could I use it later on as a positive control if the positive control stock is exhausted
21 September 2021 8,238 3 View
Hello everyone, I want to know how i can bulk fetch 3kb promoter sequences of tomato ITAG genes. Anyone has any experience?
21 September 2021 7,191 1 View
during PCR for gradient I used 55C to 68C annealing temperature. Tm of forward and reverse primers was 65.3C and 59.4C respectively. suggest me what can I do? specific product is ~436bp and non...
20 September 2021 4,704 8 View
Kindly help in this regard
18 September 2021 2,580 6 View
I have used ethidium bromide solution for genotyping different mouse strains successfully, but the laboratory that I am working on currently does not allow it. Currently, I have been using a...
16 September 2021 6,837 3 View
Hello everyone, Any recommendation on which DNA extraction kit/protocol works the best for preparing DNA samples for PacBio HiFi Sequencing? We are planning to prepare some samples from different...
12 September 2021 8,951 2 View
Can deiodinase 1 gene that codes for deiodinase enzyme, contains a sequence of noncoding transcript? If yes, what is the function of this noncoding transcript? Does noncoding transcript found...
11 September 2021 7,825 2 View
I have the reference SNP (rs) of some SNPs, how can I search to know the exact location of these SNPs on the gene? For example what is the exact location of the SNP with ID rs17716499? does it...
11 September 2021 6,268 4 View
This is my first genomics project and I have been collaborating with several other scientists. I have amplified 720 PCR products, and I am preparing to purify them and send them in for sequencing,...
09 September 2021 9,090 2 View
Hello! I want to use an old 100 mM concentrated primer for a PCR. There is probably some degradation due to time (6 years old). Thus I have quantified it in nanodrop so I could do a reliable...
09 September 2021 2,719 3 View
Hi all, I am carrying out an error prone PCR (ePCR) on a region internal to a gene that I cannot use restriction sites for. Therefore I cannot use restriction cloning to construct the library. I...
08 September 2021 5,578 1 View
Im recently working on cloning and stuck with PCR amplification and im having a hard time with snapgene and all - -the plasmid construction, the primer design Could anyone recommend me what...
08 September 2021 9,012 3 View
i have plan to add a secretion signal to my plasmid. the point is here that i do not have any source to PCR that sequence and at last do GA. i need to synthesize by myself. i want to know is it...
04 September 2021 6,317 3 View
I am developing a research project that will use 3D printed models in health education. I'm looking for 3D models of the prostate, pelvis and or male bladder.
03 September 2021 4,837 3 View
Hi there, I've amplified a beta globin gene in fragments using PCR primers. can I use same set of primers to sequence these fragments?
02 September 2021 2,525 3 View
i want to confirm my finding regarding Ancylostoma Caninum eggs by molecular studies, which is ``ITS-2 amplification``. but the number of eggs in my samples is not to much and i cant get any band...
30 August 2021 935 11 View
Hello Community! I am sending some PCR product for Sanger sequencing, which requires me to send sequencing primers along with my samples. Would the sequencing primers just be the primers I used...
30 August 2021 2,044 8 View
Hello, I am doing a microsatellite PCR and the bands appear with a few definition, the electrophoresis conditions are the following, TBE buffer 0.5X pH 8.4 (according to Sambrook, Molecular...
30 August 2021 2,832 3 View
I am trying to design PCR primers for miRNA but I am having issues getting the right primer length as well as the optimum amplicon length. I need help with blasting the primers
29 August 2021 8,872 4 View
Dear friends! I designed, checked, and ordered primers for RT PCR. How can I verify them as working now? 1. How to choose the temperature for best amplification (the primers Tm is 60,...
27 August 2021 6,670 7 View
Hey everyone, I'd like to verify if a single nucleotide variation (SNV) is in linkage disequilibrium to a 2 alleles copy number variation of 16bp (CNV). I need a program which I can input my...
26 August 2021 492 6 View
I want to clone my genes of interests in an expression vector and transform BL21 for protein expression. I was planning on using pEXP5-CT-topo-TA vector...
23 August 2021 1,072 1 View
So I did a PCR today. I've tried to amplify a quite CG rich region from a plasmid pTriex1.1. My primers should anneal and form a 666 band, instead I get a band that migrates at about 300 - 400 kb...
18 August 2021 9,084 11 View