In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
As stated, I would like to know what is the difference between NCBI blast and EzBioCloud? I have sequences of multiple unknown bacteria and am trying to do the first identification on them...
20 June 2022 7,231 4 View
Hi all, I am struggling to transform my BY4742/1 cells using the Gietz protocol along with a linear PCR fragment. I understand that using a PCR gene disruption cassette is not as efficient as...
15 June 2022 7,328 3 View
Please suggest me the best protocol for this purpose.
14 June 2022 6,520 0 View
Is it necessary to be in empty stomach of fasting( drinks) for at least 8-12 hours before lower abdomen USG? Or eating and drinking is allowed? why pressure in Urinary bladder (urination)is...
11 June 2022 8,311 10 View
I saw this structures on my primary culture of mesenchymal cells but idk what are those, does anybody knows what are those things?
29 May 2022 1,148 3 View
Hi. Can Anyone provide me with sample papers using mantel-hanzel chi square test to control for confounding? Need to see the example of how the result being reported. Thanks
23 May 2022 8,707 0 View
Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks
20 May 2022 1,287 3 View
I want to expose my lung epithelial cells to different treatments in vitro and then detect pro- and anti-inflammatory cytokines/chemokines in the conditioned media - can I then do PCRs on the...
19 May 2022 2,743 4 View
I found an exon skipping event by RT-PCR I want to assess whether exon skipping changes the reading frame? And is this variant translatable? Is there any method or software to detect it?
19 May 2022 9,512 2 View
For example, if I have a pre-post design experiment with 2 groups A and B. I can get change scores(Post minus Pre). And I can count the number of positive changes and negative changes. Hence I...
12 May 2022 8,662 3 View
Recently, we have done the macrophage antigen lpresenting experiment by co-culture of macrophages pre-loaded with OVA and CD4+/CD8+ T cells isolated from OT-II/OT-I mouse spleen. Using CFSE to...
11 May 2022 1,780 6 View
Hello, I'm trying to clone my amplified insert in pGEM-T vector using T4 DNA ligase and not getting positive clones even after trying variations in vector to insert ratio. Amplification of insert...
22 April 2022 1,039 2 View
I picked a primer sequence ,designed by the author, for my PCR from a published paper. The issue is: UCSC Insilico Pcr is not browsing that sequence, although a detailed study is done on these...
18 April 2022 8,194 6 View
Imagine a chain that is said to undergo a coil-globule transition at temperature Tc. Let's say we are just above Tc, and the chain shows a preference for a collapsed state. If the temperature were...
12 April 2022 7,707 0 View
I have outsourced Sanger sequencing of my purified PCR products. Looking at the chromatogram, I know to trim the ends. However, the ends that need to be trimmed according to the quality scores are...
08 April 2022 7,669 3 View
Dear colleagues and Gromacs users, How can I represent a radius of gyration result with a single chat on the graphic? I need to show something like Rutin as a potential inhibitor to target...
08 April 2022 3,090 0 View
I want to extract DNA from buccal swabs using proteinase K and the amount of DNA should be in higher concentration, PCR process.
07 April 2022 2,289 3 View
Why is urinary exosomes isolated at 200000 g whereas at 120000 g for cell derived exosomes as mentioned in several papers. Will not these two different speeds yield different vesicles of...
03 April 2022 9,741 0 View
Each taken gene ontology for dysregulated genes set in colorectal cancer has a biological function in neurogenesis. Can anyone explain why?
21 March 2022 3,086 3 View
As I am trying to dock protein with an organometallic ligand in autodock Vina, I'm getting some errors. So can someone guide me on how to assign a partial charge to a metal center and how to dock...
13 March 2022 5,189 1 View
if samples are limited, e.g., less than 10 samples per population, which molecular markers are suitable(Microsatellites or SNPs generated by RAD-seq)? If you can provide some references for your...
05 March 2022 2,153 4 View
How can groundwater affect forest Restoration/ aforestation?
05 March 2022 5,605 11 View
i am working on research project in which i am applying normal irrigation to tomato plants in pots experiment after three days. i have selected two other irrigation intervals 6 and 9 days. if i...
01 March 2022 1,614 3 View
I am trying to run BBmap but I am getting this error ~/bbmap$ bash stats.sh /root@wch7p10204:/home/szweda/bbmap/resources/phix174_ill.ref.fa.gz Exception in thread "main"...
09 February 2022 5,851 2 View