Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Have you applied 3D Laser scanning and Building Information Modeling for facility management during operation phase?

14 March 2020 7,806 3 View

Is there any difference between Authoritarian Mother and Authoritarian Father?

Good day! I'm looking for journal articles that would help me differentiate between an authoritarian mother and an authoritarian father. Thank you in advance!

28 February 2018 5,212 3 View

Cost-effectiveness model: Converting different rates on different rates into one probability?

Hello Everyone, Need quick help! I have 5 different rates for recurrence from 5 studies with different followup. How do I combine them to find one probability to be used in cost-effectiveness...

05 November 2017 4,107 7 View

Are there known antibodies produced when a human is infected with STH (Ascaris lumbricoides, Trichuris trichiura, hookworm)?

Are there known antibodies produced when humans are infected with STH (Ascaris lumbricoides, Trichuris trichiura, hookworm) and do the produced antibodies differ per helminth? Thanks in advance!

19 September 2017 1,116 5 View

How long we can keep soil water extraction in refrigerator (4oC) ?

07 August 2017 6,149 6 View

Is there an assay that is able to quantify the degradative enzymatic activity of fungi without isolating the enzyme from the fungi?

18 May 2017 8,694 3 View

What are the implications of neutral theory for interpreting patterns of genetic variation in ecology and evolution?

04 November 2016 7,331 4 View

Any changes in soil dissolved organic matter if we conduct second time extraction by hot/cold water?

06 July 2016 8,229 3 View

Any changes in soil dissolved organic matter if we conduct second time extraction by hot/cold water?

05 July 2016 2,288 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Is there any correlation between mature miRNAs and their precursors?

I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...

07 December 2020 7,744 3 View

Hello every one! How can i design the primer for mature microRNA for RT-PCR quantification?

I am going to validate some microRNA genes in two developmental stages. But i found the method for miRNA primer designing is totally change compering to mRNA and LncRNA primers designing. Please...

03 December 2020 4,555 6 View

What type of cellular structures are these likely to be?

I have immunohistochemistry slides of human subcutaneous fat biopsy, with skin attached. Most of the structures are self-evident - skin, adipocytes, microvessels in transverse section (revealed by...

03 December 2020 4,238 7 View

Help in Sample Size Calculation for my Thesis Comparing the Clinical Outcome of Pulpotomy in Mature Permanent Teeth with MTA & EBRRM?

I am looking for assistance in finding statistics for sample size calculation on topic Comparing the Clinical Outcome of Complete Pulpotomy in Mature Permanent Teeth using MTA & Endosequence...

20 October 2020 8,197 6 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View