After analyzing the qPCR data, I found that the control fold change averages are 1.8, 2.5, and 1.2. Typically, discussions suggest that the control fold change average should be 1, but in my case, it is higher. My calculations are precise, and the duplicates are nearly identical, with no significant differences.

Is there something wrong with my calculations, or can the control fold change be more than 1? I would appreciate expert opinions on this matter and any other methods to validate my calculations. Thank you.