For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm is 2592. can we use the following formula for beta-carotene quantification? if yes, please share some reference.
Beta-carotene (mg/100g)= (Absorbance* total volume * 100)/( 0.2592* weight of sample *1000)
Paul Körner
No, you have to apply the Beer–Lambert law. (https://en.wikipedia.org/wiki/Beer%E2%80%93Lambert_law) However, this is only possible in a certain absorbance range (0.2 - 1.2, I think). Also, extinction coefficients found in literature are not sufficiently relieable. You have to determine it yourself by a calibration.
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